Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4419564..4420399 Replicon chromosome
Accession NZ_CP101925
Organism Escherichia coli strain Seattle 1946

Toxin (Protein)


Gene name yeeV Uniprot ID A0A0J2AEA6
Locus tag NP429_RS21350 Protein ID WP_000854759.1
Coordinates 4419564..4419941 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1NM52
Locus tag NP429_RS21355 Protein ID WP_001295723.1
Coordinates 4420031..4420399 (-) Length 123 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NP429_RS21325 (4415675) 4415675..4417297 - 1623 WP_001295726.1 Alw26I/Eco31I/Esp3I family type II restriction adenine-specific DNA-methyltransferase -
NP429_RS21330 (4418088) 4418088..4418264 - 177 Protein_4182 helix-turn-helix domain-containing protein -
NP429_RS21335 (4418631) 4418631..4418780 - 150 Protein_4183 hypothetical protein -
NP429_RS21340 (4418886) 4418886..4419062 - 177 WP_000839286.1 DUF957 domain-containing protein -
NP429_RS21345 (4419079) 4419079..4419567 - 489 WP_000761690.1 DUF5983 family protein -
NP429_RS21350 (4419564) 4419564..4419941 - 378 WP_000854759.1 TA system toxin CbtA family protein Toxin
NP429_RS21355 (4420031) 4420031..4420399 - 369 WP_001295723.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
NP429_RS21360 (4420562) 4420562..4420783 - 222 WP_000692345.1 DUF987 domain-containing protein -
NP429_RS21365 (4420846) 4420846..4421322 - 477 WP_001186775.1 RadC family protein -
NP429_RS21370 (4421338) 4421338..4421811 - 474 WP_001350782.1 antirestriction protein -
NP429_RS21375 (4422153) 4422153..4422971 - 819 WP_001234738.1 DUF932 domain-containing protein -
NP429_RS21380 (4423089) 4423089..4423284 - 196 Protein_4192 DUF905 family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4408023..4434935 26912


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-112)

Antitoxin

(18-117)


Sequences


Toxin        


Download         Length: 126 a.a.        Molecular weight: 14144.26 Da        Isoelectric Point: 7.3249

>T253005 WP_000854759.1 NZ_CP101925:c4419941-4419564 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Download         Length: 378 bp


Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13592.40 Da        Isoelectric Point: 6.6255

>AT253005 WP_001295723.1 NZ_CP101925:c4420399-4420031 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Download         Length: 369 bp


Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0J2AEA6


Antitoxin

Source ID Structure
AlphaFold DB S1NM52

References