Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4337502..4338078 Replicon chromosome
Accession NZ_CP101925
Organism Escherichia coli strain Seattle 1946

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag NP429_RS20950 Protein ID WP_001295743.1
Coordinates 4337791..4338078 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag NP429_RS20945 Protein ID WP_000063148.1
Coordinates 4337502..4337804 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NP429_RS20930 (4334098) 4334098..4336248 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
NP429_RS20935 (4336298) 4336298..4336501 + 204 WP_000467859.1 YbdD/YjiX family protein -
NP429_RS20940 (4336512) 4336512..4337468 + 957 WP_001295745.1 GTPase -
NP429_RS20945 (4337502) 4337502..4337804 - 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
NP429_RS20950 (4337791) 4337791..4338078 - 288 WP_001295743.1 BrnT family toxin Toxin
NP429_RS20955 (4338275) 4338275..4339441 + 1167 WP_000800831.1 restriction endonuclease -
NP429_RS20960 (4339505) 4339505..4341124 + 1620 WP_001029745.1 class I SAM-dependent DNA methyltransferase -
NP429_RS20965 (4341114) 4341114..4342418 + 1305 WP_000535012.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T253004 WP_001295743.1 NZ_CP101925:c4338078-4337791 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT253004 WP_000063148.1 NZ_CP101925:c4337804-4337502 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References