Detailed information of TA system TA025300

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 1838508..1839073
• Replicon: chromosome
• Accession: NC_015577
• Organism: Treponema azotonutricium ZAS-9

Toxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: TREAZ_RS07915
• Protein ID: WP_015711308.1
• Coordinates: 1838508..1838837 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazF
• Uniprot ID: F5YAX7
• Locus tag: TREAZ_RS07920
• Protein ID: WP_015711309.1
• Coordinates: 1838825..1839073 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TREAZ_RS07900	1834641..1837397	-	2757	WP_015711305.1	preprotein translocase subunit SecA	-
TREAZ_RS07905	1837484..1837810	-	327	WP_043922990.1	hypothetical protein	-
TREAZ_RS07910	1837977..1838258	-	282	WP_043922991.1	UPF0175 family protein	-
TREAZ_RS07915	1838508..1838837	-	330	WP_015711308.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
TREAZ_RS07920	1838825..1839073	-	249	WP_015711309.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
TREAZ_RS07925	1839239..1840048	+	810	WP_015711311.1	Rpn family recombination-promoting nuclease/putative transposase	-
TREAZ_RS07930	1840118..1841098	+	981	WP_015711312.1	extracellular solute-binding protein	-
TREAZ_RS07935	1841086..1841874	+	789	WP_015711313.1	ABC transporter ATP-binding protein	-
TREAZ_RS07940	1841886..1843454	+	1569	WP_015711314.1	ABC transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-106)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12863.27 Da
• Isoelectric Point: 9.3988

>T25300 WP_015711308.1 NC_015577:c1838837-1838508 [Treponema azotonutricium ZAS-9]
MGMVMKQYEIYLVNLEPTIRHEIQKTRPCVIVSPDEMNEHIETIIIAPMTKKSYNYPTRIEINFQSKTGWIVLDQIRTVD
KKRLIRKIGELDKSKATEIKDIIMEMLVK

Nucleotide

• Length: 330 bp

>T25300 NC_015577:c1838837-1838508 [Treponema azotonutricium ZAS-9]
ATGGGAATGGTGATGAAACAGTATGAAATTTATCTGGTAAATCTTGAACCAACAATAAGGCATGAAATACAAAAAACACG
TCCCTGTGTAATAGTTTCTCCCGATGAAATGAATGAACATATAGAAACGATAATAATTGCGCCAATGACGAAAAAGTCTT
ATAACTATCCAACAAGGATAGAAATTAATTTTCAAAGCAAAACAGGCTGGATTGTCTTGGATCAAATAAGGACAGTAGAT
AAAAAAAGATTAATCAGGAAAATTGGCGAACTGGATAAAAGCAAGGCAACTGAAATAAAAGACATTATTATGGAAATGCT
GGTAAAATAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9717.09 Da
• Isoelectric Point: 4.6631

>AT25300 WP_015711309.1 NC_015577:c1839073-1838825 [Treponema azotonutricium ZAS-9]
MLVSVVPIGNSKGIRIPKNILTQLNIENEVEMNMHNNEIIIKRIEKKPRSGWEEAFQKMHKEGDDNLLLQEQIQDDSFEW
EW

Nucleotide

• Length: 249 bp

>AT25300 NC_015577:c1839073-1838825 [Treponema azotonutricium ZAS-9]
ATGCTTGTTTCTGTGGTGCCAATCGGAAATTCAAAGGGAATCAGGATACCAAAAAATATTCTAACTCAACTTAATATTGA
AAACGAGGTTGAAATGAATATGCATAACAACGAAATTATAATAAAACGCATTGAGAAAAAACCTCGGAGCGGCTGGGAAG
AAGCATTTCAAAAAATGCACAAAGAAGGAGACGATAATCTGCTTCTTCAAGAACAAATTCAAGATGATTCTTTTGAATGG
GAATGGTGA

Structures

Antitoxin

• AlphaFold DB: F5YAX7