Detailed information of TA system TA025299

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 404199..404884
• Replicon: chromosome
• Accession: NC_015577
• Organism: Treponema azotonutricium ZAS-9

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: F5YAN8
• Locus tag: TREAZ_RS01760
• Protein ID: WP_015710070.1
• Coordinates: 404462..404884 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: TREAZ_RS01755
• Protein ID: WP_043922711.1
• Coordinates: 404199..404465 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TREAZ_RS01730	399357..400457	+	1101	WP_015710065.1	endonuclease/exonuclease/phosphatase family protein	-
TREAZ_RS01735	400460..401221	-	762	WP_015710066.1	DeoR/GlpR family DNA-binding transcription regulator	-
TREAZ_RS01740	401424..401948	-	525	WP_015710067.1	nitroreductase family protein	-
TREAZ_RS01745	401992..403776	-	1785	WP_015710068.1	threonine--tRNA ligase	-
TREAZ_RS01755	404199..404465	+	267	WP_043922711.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
TREAZ_RS01760	404462..404884	+	423	WP_015710070.1	type II toxin-antitoxin system VapC family toxin	Toxin
TREAZ_RS01765	404894..405463	-	570	WP_015710071.1	SIS domain-containing protein	-
TREAZ_RS01770	405501..408635	-	3135	WP_148257855.1	FtsX-like permease family protein	-
TREAZ_RS01775	408652..409353	-	702	WP_015710073.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (10-68)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15871.63 Da
• Isoelectric Point: 7.0615

>T25299 WP_015710070.1 NC_015577:404462-404884 [Treponema azotonutricium ZAS-9]
MKYLLDTNVISEIRKRNSNPRVLSYMRNIPIEDLFLSVVCIGEIAKGIEKLPLGKKKEELAYWLDSAVPEQFADCILPLD
SECMQEWGRMCAKSKRTLPVLDSLIAATALVHRLILLTRNISDFEDIPGLSCISPWNGKH

Nucleotide

• Length: 423 bp

>T25299 NC_015577:404462-404884 [Treponema azotonutricium ZAS-9]
ATGAAGTATCTCCTGGATACCAATGTCATATCTGAAATCCGCAAAAGGAATAGCAATCCACGGGTACTCTCATACATGCG
GAATATCCCCATTGAGGATCTTTTTCTTAGCGTTGTTTGTATCGGGGAAATTGCCAAAGGAATAGAAAAACTCCCCTTGG
GGAAAAAGAAAGAAGAATTAGCCTACTGGCTCGACTCTGCCGTGCCGGAACAGTTTGCCGATTGTATTCTTCCGCTTGAT
TCAGAGTGCATGCAGGAATGGGGCCGTATGTGCGCCAAATCCAAACGGACGCTACCCGTCCTCGACTCGCTAATTGCCGC
TACCGCCCTGGTCCATCGCTTGATTTTGCTCACACGAAATATCTCTGATTTTGAGGATATCCCGGGACTAAGCTGCATCA
GCCCATGGAATGGTAAACACTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9931.55 Da
• Isoelectric Point: 10.1039

>AT25299 WP_043922711.1 NC_015577:404199-404465 [Treponema azotonutricium ZAS-9]
MQTLKKKTCWQLQEAKAQLSQVIRNAATSPQIITLRGEEAAVILSTEQYNKLTKPKMGVVEFFQNSPLADVAIDLKRDKS
TEIRTINL

Nucleotide

• Length: 267 bp

>AT25299 NC_015577:404199-404465 [Treponema azotonutricium ZAS-9]
ATGCAGACCTTGAAGAAAAAGACTTGTTGGCAGCTCCAAGAGGCTAAGGCTCAACTTAGTCAGGTTATCAGAAATGCAGC
CACCTCACCGCAGATCATTACGCTGCGGGGAGAGGAGGCGGCAGTAATTCTATCCACAGAGCAGTACAACAAACTGACCA
AACCAAAGATGGGAGTTGTTGAATTTTTTCAGAATTCTCCATTGGCAGATGTGGCAATAGATTTGAAACGGGATAAGTCC
ACCGAGATCAGGACGATTAATTTATGA

Structures

Toxin

• AlphaFold DB: F5YAN8