Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataT-KacA/DUF1778(antitoxin)
Location 36819..37617 Replicon plasmid pEcFELIX526.1
Accession NZ_CP101923
Organism Escherichia coli strain JCC-EE 7

Toxin (Protein)


Gene name ataT Uniprot ID A0A0K4UCL9
Locus tag NP442_RS22855 Protein ID WP_024173399.1
Coordinates 37096..37617 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A0V9R6Q7
Locus tag NP442_RS22850 Protein ID WP_001351987.1
Coordinates 36819..37088 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NP442_RS22825 (NP442_22820) 32359..33699 - 1341 WP_000137333.1 DnaB-like helicase C-terminal domain-containing protein -
NP442_RS22830 (NP442_22825) 33743..34483 - 741 WP_021520120.1 hypothetical protein -
NP442_RS22835 (NP442_22830) 34673..35428 - 756 WP_085674543.1 DUF2971 domain-containing protein -
NP442_RS22840 (NP442_22835) 35473..35826 - 354 WP_164715282.1 hypothetical protein -
NP442_RS22845 (NP442_22840) 35832..36500 - 669 WP_000161228.1 division plane positioning ATPase MipZ -
NP442_RS22850 (NP442_22845) 36819..37088 + 270 WP_001351987.1 DUF1778 domain-containing protein Antitoxin
NP442_RS22855 (NP442_22850) 37096..37617 + 522 WP_024173399.1 GNAT family N-acetyltransferase Toxin
NP442_RS22860 (NP442_22855) 37785..38036 - 252 WP_001404395.1 hypothetical protein -
NP442_RS22865 (NP442_22860) 38038..38730 - 693 WP_000856757.1 hypothetical protein -
NP442_RS22870 (NP442_22865) 38744..39067 - 324 WP_000064175.1 hypothetical protein -
NP442_RS22875 (NP442_22870) 39390..39956 - 567 WP_226438823.1 hypothetical protein -
NP442_RS22880 (NP442_22875) 39998..40252 - 255 WP_000120169.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-15 - 1..111928 111928


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19475.31 Da        Isoelectric Point: 8.6344

>T252984 WP_024173399.1 NZ_CP101923:37096-37617 [Escherichia coli]
VDNIKIEIFSGEKDYDLNGFDCGEESLNAFLTNHLKRQHEGKILRAYVLCTKEGRPKVLGYYTLSGSCFEKESLPSRSQQ
KKVPYRNVPSVTLGRLALDKSLQGQGFGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKAKAFYKSLDFIQLVGNNERSL
FYPTKSIEKLFEE

Download         Length: 522 bp

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10082.54 Da        Isoelectric Point: 7.0155

>AT252984 WP_001351987.1 NZ_CP101923:36819-37088 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLNEESWNLVMDAISNPPAPNDKLKR
AADRLKSME

Download         Length: 270 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K4UCL9

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9R6Q7

References