Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3916307..3916883 Replicon chromosome
Accession NZ_CP101922
Organism Escherichia coli strain JCC-EE 7

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag NP442_RS19250 Protein ID WP_001297643.1
Coordinates 3916596..3916883 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag NP442_RS19245 Protein ID WP_000063156.1
Coordinates 3916307..3916609 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NP442_RS19230 (3912903) 3912903..3915053 + 2151 WP_001736090.1 pyruvate/proton symporter BtsT -
NP442_RS19235 (3915103) 3915103..3915306 + 204 WP_000467859.1 YbdD/YjiX family protein -
NP442_RS19240 (3915317) 3915317..3916273 + 957 WP_001297640.1 GTPase -
NP442_RS19245 (3916307) 3916307..3916609 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
NP442_RS19250 (3916596) 3916596..3916883 - 288 WP_001297643.1 BrnT family toxin Toxin
NP442_RS19255 (3917082) 3917082..3917779 - 698 Protein_3762 IS1 family transposase -
NP442_RS19260 (3917836) 3917836..3917931 + 96 Protein_3763 dienelactone hydrolase family protein -
NP442_RS19265 (3918077) 3918077..3919309 + 1233 WP_106425455.1 multidrug efflux MFS transporter MdtM -
NP442_RS19270 (3919350) 3919350..3920630 + 1281 WP_074480746.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T252983 WP_001297643.1 NZ_CP101922:c3916883-3916596 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT252983 WP_000063156.1 NZ_CP101922:c3916609-3916307 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References