Detailed information of TA system TA025295

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 2228597..2229150
• Replicon: chromosome
• Accession: NC_015576
• Organism: Mycobacterium sp. JDM601

Toxin (Protein)

• Gene name: relE
• Uniprot ID: F5YSQ6
• Locus tag: JDM601_RS10385
• Protein ID: WP_013828990.1
• Coordinates: 2228597..2228860 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: JDM601_RS10390
• Protein ID: WP_041319839.1
• Coordinates: 2228869..2229150 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JDM601_RS10345	2224056..2224664	+	609	WP_041317901.1	hypothetical protein	-
JDM601_RS10350	2224856..2225095	+	240	WP_041317904.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
JDM601_RS10355	2225122..2225520	+	399	WP_041317907.1	type II toxin-antitoxin system VapC family toxin	-
JDM601_RS10360	2225563..2225796	+	234	WP_041317910.1	hypothetical protein	-
JDM601_RS10365	2225793..2226128	+	336	WP_041317913.1	type II toxin-antitoxin system PemK/MazF family toxin	-
JDM601_RS10370	2226339..2226548	-	210	WP_013828986.1	hypothetical protein	-
JDM601_RS10375	2226749..2227672	+	924	WP_041317916.1	aromatic alcohol reductase	-
JDM601_RS23360	2227721..2227867	+	147	WP_013828988.1	hypothetical protein	-
JDM601_RS10380	2227877..2228165	+	289	Protein_2069	DUF1778 domain-containing protein	-
JDM601_RS10385	2228597..2228860	-	264	WP_013828990.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JDM601_RS10390	2228869..2229150	-	282	WP_041319839.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JDM601_RS10395	2230077..2231318	-	1242	WP_013828992.1	IS256 family transposase	-
JDM601_RS22225	2231456..2231896	+	441	WP_081465522.1	TnsA-like heteromeric transposase endonuclease subunit	-
JDM601_RS10405	2232019..2233977	+	1959	WP_041319841.1	DDE-type integrase/transposase/recombinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2230077..2231318	1241

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10198.80 Da
• Isoelectric Point: 11.7303

>T25295 WP_013828990.1 NC_015576:c2228860-2228597 [Mycolicibacter sinensis]
MGYTVRFTSTARRDLHKLPPRVLSAVIEFAFGDLAHEPHRVGKPLRRELTGLYSARRGPYRLLYRIDQGRSLVPIQRVDH
RADVYRR

Nucleotide

• Length: 264 bp

>T25295 NC_015576:c2228860-2228597 [Mycolicibacter sinensis]
ATGGGTTACACGGTTAGGTTCACCAGCACCGCGCGGCGCGACCTGCACAAGCTGCCGCCACGGGTGCTCTCGGCCGTGAT
TGAGTTCGCTTTCGGCGACCTGGCACACGAACCGCACCGCGTTGGCAAGCCGCTCCGGCGTGAACTGACCGGGCTGTATA
GCGCCCGGCGCGGACCGTACCGCCTGTTGTACCGGATCGACCAGGGGCGCTCACTGGTGCCGATTCAGCGTGTCGATCAT
CGCGCGGACGTTTACCGACGCTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10183.37 Da
• Isoelectric Point: 4.1849

>AT25295 WP_041319839.1 NC_015576:c2229150-2228869 [Mycolicibacter sinensis]
MRILAISKMKDKLNEYVDAVAQTQDQITITKNGAPAAVLVGADEWESLQETLYWLSQPGIKESIAEADADIDAGRTYGED
EIRAEFGAPRRAG

Nucleotide

• Length: 282 bp

>AT25295 NC_015576:c2229150-2228869 [Mycolicibacter sinensis]
ATGCGGATACTGGCCATCTCGAAGATGAAGGACAAGCTCAACGAGTACGTCGATGCGGTCGCCCAAACGCAGGATCAGAT
CACGATCACCAAGAACGGTGCACCGGCGGCGGTGCTCGTCGGCGCCGACGAGTGGGAATCGTTGCAAGAGACGCTCTACT
GGCTTTCCCAGCCCGGGATCAAAGAATCGATTGCTGAGGCCGACGCCGATATTGACGCTGGGCGCACGTACGGCGAGGAC
GAAATTCGTGCCGAGTTCGGCGCGCCTCGGCGTGCCGGCTAA

Structures

Toxin

• AlphaFold DB: F5YSQ6