Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4599176..4599673 Replicon chromosome
Accession NZ_CP101920
Organism Escherichia coli strain QA-1986 630

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag NP447_RS22545 Protein ID WP_001173343.1
Coordinates 4599176..4599349 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag NP447_RS22550 Protein ID WP_000398619.1
Coordinates 4599377..4599673 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NP447_RS22535 (4595846) 4595846..4597303 + 1458 WP_000856832.1 dipeptide/tripeptide permease DtpC -
NP447_RS22540 (4597540) 4597540..4599057 + 1518 WP_001295074.1 lysine--tRNA ligase -
NP447_RS22545 (4599176) 4599176..4599349 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
NP447_RS22550 (4599377) 4599377..4599673 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
NP447_RS22555 (4599900) 4599900..4600172 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
NP447_RS22560 (4600184) 4600184..4600414 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
NP447_RS22565 (4600595) 4600595..4602226 + 1632 WP_114747508.1 sensor histidine kinase -
NP447_RS22570 (4602223) 4602223..4602942 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T252942 WP_001173343.1 NZ_CP101920:c4599349-4599176 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT252942 WP_000398619.1 NZ_CP101920:c4599673-4599377 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References