Detailed information of TA system TA025293

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1947703..1948343
• Replicon: chromosome
• Accession: NC_015576
• Organism: Mycobacterium sp. JDM601

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JDM601_RS09065
• Protein ID: WP_013828728.1
• Coordinates: 1947703..1948092 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F5Z1W7
• Locus tag: JDM601_RS09070
• Protein ID: WP_013828729.1
• Coordinates: 1948089..1948343 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JDM601_RS09040	1944019..1944438	-	420	WP_013828723.1	hypothetical protein	-
JDM601_RS09045	1944632..1945183	+	552	WP_158019570.1	TetR/AcrR family transcriptional regulator	-
JDM601_RS09050	1945277..1945444	+	168	WP_158019458.1	hypothetical protein	-
JDM601_RS09055	1945462..1946109	+	648	WP_081465647.1	DUF2293 domain-containing protein	-
JDM601_RS09060	1946140..1947648	+	1509	WP_013828727.1	proteasome accessory factor PafA2	-
JDM601_RS09065	1947703..1948092	-	390	WP_013828728.1	PIN domain-containing protein	Toxin
JDM601_RS09070	1948089..1948343	-	255	WP_013828729.1	hypothetical protein	Antitoxin
JDM601_RS09075	1948531..1948725	+	195	WP_013828730.1	ubiquitin-like protein Pup	-
JDM601_RS09080	1948722..1949606	+	885	WP_041317760.1	proteasome subunit beta	-
JDM601_RS09085	1949603..1950349	+	747	WP_013828732.1	proteasome subunit alpha	-
JDM601_RS09090	1950358..1950594	-	237	WP_013828733.1	DUF167 domain-containing protein	-
JDM601_RS09095	1950637..1950888	+	252	WP_013828734.1	hypothetical protein	-
JDM601_RS09100	1950895..1951326	+	432	WP_013828735.1	type II toxin-antitoxin system VapC family toxin	-
JDM601_RS09105	1951413..1952615	-	1203	WP_013828736.1	lipid-transfer protein	-
JDM601_RS09110	1952612..1953055	-	444	WP_041319776.1	OB-fold domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (3-118)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 13896.73 Da
• Isoelectric Point: 4.7729

>T25293 WP_013828728.1 NC_015576:c1948092-1947703 [Mycolicibacter sinensis]
MIVVDAAALVDALTGVDGTDELRSELADEELHAPALLDFEVVSALRGLTRGGQLSAARAHDALADFDALAIRRWPGSAEL
RLRAFTLRDNLTAYDAAYVALAEALECAVLTRDRRLARSGGHSVEIRVR

Nucleotide

• Length: 390 bp

>T25293 NC_015576:c1948092-1947703 [Mycolicibacter sinensis]
ATGATCGTCGTCGATGCCGCAGCGCTCGTCGACGCGCTCACCGGCGTCGACGGCACCGACGAACTGCGCAGTGAACTCGC
CGACGAGGAACTACATGCCCCGGCGTTGTTGGACTTCGAAGTCGTCTCCGCGTTGCGTGGCCTGACCCGCGGCGGGCAGC
TCAGCGCGGCACGGGCGCACGACGCCCTGGCCGACTTCGATGCGCTGGCCATCCGGCGGTGGCCCGGTAGCGCGGAACTT
CGGCTGCGGGCGTTCACGCTGCGCGACAATCTGACGGCCTACGACGCGGCTTATGTGGCGCTCGCCGAGGCGTTGGAATG
CGCTGTGCTGACCCGTGACCGGCGACTGGCGCGCTCGGGTGGTCACAGCGTAGAGATCCGTGTCCGCTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9258.50 Da
• Isoelectric Point: 10.3629

>AT25293 WP_013828729.1 NC_015576:c1948343-1948089 [Mycolicibacter sinensis]
MSSLLQIRNVPDAVRRELKARAAIQGQSLNAYLLDLISREVNRPTVAEVLQRAAQRAEHASGSAIDVVDADRIERDAQLA
GPRR

Nucleotide

• Length: 255 bp

>AT25293 NC_015576:c1948343-1948089 [Mycolicibacter sinensis]
ATGAGTTCGCTACTTCAGATCCGCAATGTTCCCGACGCGGTTCGTCGGGAACTCAAGGCGCGGGCGGCCATACAGGGCCA
GTCACTGAACGCCTACCTGCTCGACCTGATCAGCCGGGAAGTGAACCGCCCCACAGTCGCCGAAGTCCTGCAGCGCGCCG
CGCAACGCGCCGAGCATGCCAGCGGGTCGGCGATCGATGTAGTCGACGCCGACCGAATCGAGCGGGATGCGCAATTGGCC
GGCCCCCGCAGATGA

Structures

Antitoxin

• AlphaFold DB: A0A7I9Y7W3