Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1000141..1000795 Replicon chromosome
Accession NZ_CP101920
Organism Escherichia coli strain QA-1986 630

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag NP447_RS04835 Protein ID WP_000244781.1
Coordinates 1000388..1000795 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag NP447_RS04830 Protein ID WP_000354046.1
Coordinates 1000141..1000407 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NP447_RS04805 (995310) 995310..996053 + 744 WP_000951961.1 SDR family oxidoreductase -
NP447_RS04810 (996110) 996110..997543 - 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
NP447_RS04815 (997588) 997588..997899 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
NP447_RS04820 (998063) 998063..998722 + 660 WP_000250274.1 hemolysin III family protein -
NP447_RS04825 (998918) 998918..999898 - 981 WP_000886095.1 tRNA-modifying protein YgfZ -
NP447_RS04830 (1000141) 1000141..1000407 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
NP447_RS04835 (1000388) 1000388..1000795 + 408 WP_000244781.1 protein YgfX Toxin
NP447_RS04840 (1000835) 1000835..1001356 - 522 WP_001055874.1 flavodoxin FldB -
NP447_RS04845 (1001468) 1001468..1002364 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
NP447_RS04850 (1002389) 1002389..1003099 + 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
NP447_RS04855 (1003105) 1003105..1004838 + 1734 WP_000813218.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T252929 WP_000244781.1 NZ_CP101920:1000388-1000795 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT252929 WP_000354046.1 NZ_CP101920:1000141-1000407 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References