Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3990042..3990618 Replicon chromosome
Accession NZ_CP101915
Organism Escherichia coli strain Evo1

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag NP443_RS19360 Protein ID WP_001297643.1
Coordinates 3990331..3990618 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag NP443_RS19355 Protein ID WP_000063160.1
Coordinates 3990042..3990344 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NP443_RS19340 (3986638) 3986638..3988788 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
NP443_RS19345 (3988838) 3988838..3989041 + 204 WP_000467859.1 YbdD/YjiX family protein -
NP443_RS19350 (3989052) 3989052..3990008 + 957 WP_001297640.1 GTPase -
NP443_RS19355 (3990042) 3990042..3990344 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
NP443_RS19360 (3990331) 3990331..3990618 - 288 WP_001297643.1 BrnT family toxin Toxin
NP443_RS19365 (3990817) 3990817..3991513 - 697 Protein_3784 IS1 family transposase -
NP443_RS19370 (3991828) 3991828..3993060 + 1233 WP_001137030.1 multidrug efflux MFS transporter MdtM -
NP443_RS19375 (3993101) 3993101..3994381 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3990817..3991026 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T252920 WP_001297643.1 NZ_CP101915:c3990618-3990331 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT252920 WP_000063160.1 NZ_CP101915:c3990344-3990042 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References