Detailed information of TA system TA025292

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 1350086..1350737
• Replicon: chromosome
• Accession: NC_015576
• Organism: Mycobacterium sp. JDM601

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: F5YWI3
• Locus tag: JDM601_RS06380
• Protein ID: WP_013828211.1
• Coordinates: 1350303..1350737 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: JDM601_RS06375
• Protein ID: WP_080575708.1
• Coordinates: 1350086..1350319 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JDM601_RS06370	1348755..1350050	+	1296	WP_158019450.1	DUF2399 domain-containing protein	-
JDM601_RS06375	1350086..1350319	+	234	WP_080575708.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JDM601_RS06380	1350303..1350737	+	435	WP_013828211.1	type II toxin-antitoxin system VapC family toxin	Toxin
JDM601_RS06385	1350852..1351358	-	507	WP_013828212.1	histidine phosphatase family protein	-
JDM601_RS06390	1351592..1352737	+	1146	WP_013828213.1	exonuclease SbcCD subunit D	-
JDM601_RS06395	1352734..1355349	+	2616	WP_013828214.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-125)

Antitoxin

• SpoVT-AbrB domain (2-37)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15621.11 Da
• Isoelectric Point: 10.2190

>T25292 WP_013828211.1 NC_015576:1350303-1350737 [Mycolicibacter sinensis]
MPTNAEHVLVDTSAALALAQRESLFHHAAKARLLACRRGMAGHAAVELLSVLTRLPPPQRLSPVAALRLQETNFPESRFL
SATDTKNLLREFAEVGLAGGALYDALVGAAARKHKLPLISCDRRAEPTYRVLGVNHELLSPLSR

Nucleotide

• Length: 435 bp

>T25292 NC_015576:1350303-1350737 [Mycolicibacter sinensis]
TTGCCGACCAACGCTGAGCACGTACTGGTCGACACCAGCGCCGCACTGGCACTGGCACAGCGTGAGAGTCTGTTTCACCA
TGCCGCCAAGGCTCGACTGCTGGCCTGTCGTCGTGGGATGGCCGGGCATGCCGCGGTGGAGCTGCTGTCGGTGCTCACCC
GATTGCCCCCACCGCAGCGCCTGAGTCCGGTTGCCGCCCTTCGTCTGCAAGAGACGAATTTTCCGGAGTCTCGATTCCTG
TCGGCAACTGACACCAAGAACCTCTTGCGGGAGTTCGCCGAAGTGGGCCTGGCCGGCGGAGCGCTCTACGACGCTCTGGT
CGGCGCGGCGGCCCGCAAGCATAAGCTGCCGCTCATCAGCTGCGACCGACGGGCTGAACCGACATATCGCGTGCTCGGCG
TCAACCACGAATTGCTATCGCCGCTTTCGCGGTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8253.29 Da
• Isoelectric Point: 4.0834

>AT25292 WP_080575708.1 NC_015576:1350086-1350319 [Mycolicibacter sinensis]
MRTTIDNAGRVVIPKALRERSGITPGEVEISVDGAGVRIESVAVDELLEEDGLLLLPDGGTELDADAVRELRLADQR

Nucleotide

• Length: 234 bp

>AT25292 NC_015576:1350086-1350319 [Mycolicibacter sinensis]
ATGCGCACTACCATCGATAATGCCGGACGCGTGGTAATACCGAAGGCGCTTCGTGAGCGGTCGGGTATTACCCCCGGTGA
GGTGGAGATATCGGTCGACGGGGCCGGCGTTCGTATCGAAAGCGTGGCAGTCGACGAGCTCCTCGAAGAGGATGGGCTTC
TGCTGCTGCCCGATGGTGGCACAGAGCTCGACGCCGACGCGGTGCGGGAGTTGCGGCTTGCCGACCAACGCTGA

Structures

Toxin

• AlphaFold DB: A0A1B8SDU3