Detailed information of TA system TA025291

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 606475..607154
• Replicon: chromosome
• Accession: NC_015576
• Organism: Mycobacterium sp. JDM601

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: F5Z2H2
• Locus tag: JDM601_RS02895
• Protein ID: WP_013827526.1
• Coordinates: 606723..607154 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: JDM601_RS02890
• Protein ID: WP_019736076.1
• Coordinates: 606475..606726 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JDM601_RS02850	601698..601991	+	294	WP_013827517.1	hypothetical protein	-
JDM601_RS02855	602099..602878	+	780	WP_013827518.1	hypothetical protein	-
JDM601_RS02860	602879..603226	-	348	WP_013827519.1	hypothetical protein	-
JDM601_RS02865	603240..603533	-	294	Protein_573	hypothetical protein	-
JDM601_RS02870	603592..603900	-	309	WP_041316980.1	hypothetical protein	-
JDM601_RS02875	603900..604250	-	351	WP_041316983.1	hypothetical protein	-
JDM601_RS21715	604271..605506	-	1236	WP_013827523.1	DUF4226 domain-containing protein	-
JDM601_RS02885	605559..606335	-	777	WP_193374438.1	thioesterase family protein	-
JDM601_RS02890	606475..606726	+	252	WP_019736076.1	ribbon-helix-helix domain-containing protein	Antitoxin
JDM601_RS02895	606723..607154	+	432	WP_013827526.1	type II toxin-antitoxin system VapC family toxin	Toxin
JDM601_RS02900	607356..608201	-	846	WP_013827528.1	methyltransferase domain-containing protein	-
JDM601_RS02905	608243..609019	+	777	WP_013827529.1	thioesterase family protein	-
JDM601_RS02910	609035..609397	-	363	WP_013827530.1	DoxX family protein	-
JDM601_RS02915	609709..610125	+	417	WP_013827531.1	hypothetical protein	-
JDM601_RS02920	610300..610557	+	258	WP_158019424.1	hypothetical protein	-
JDM601_RS02930	611045..612040	+	996	WP_013827533.1	metallophosphoesterase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15006.07 Da
• Isoelectric Point: 4.8989

>T25291 WP_013827526.1 NC_015576:606723-607154 [Mycolicibacter sinensis]
MIVLDTTVLVYAKGADHRFREPCRDLIAAVAADRIEATTTPEVIQEFVHIRARRRGRNDAATLGRSYAELLAPLLTVAGD
HLERGLTMFETTPSVGAVDSVLAAVAKSAGAVLVSADGAFSDLPDITHVVPDTAGVAGLLGDN

Nucleotide

• Length: 432 bp

>T25291 NC_015576:606723-607154 [Mycolicibacter sinensis]
ATGATCGTGCTGGACACCACGGTGCTCGTCTATGCCAAGGGCGCCGACCACCGGTTCAGGGAGCCGTGCCGTGACTTGAT
CGCGGCGGTTGCCGCCGACCGCATTGAAGCGACCACTACCCCCGAAGTGATCCAGGAGTTCGTCCATATTCGTGCCCGCC
GACGTGGCCGCAACGATGCCGCAACGCTGGGGCGCAGCTACGCCGAGCTGCTCGCGCCCCTGCTGACTGTCGCTGGCGAC
CACCTCGAGCGAGGATTGACGATGTTCGAGACGACCCCGAGTGTTGGTGCCGTTGATTCCGTGCTGGCTGCGGTCGCCAA
ATCTGCCGGGGCGGTACTGGTCTCGGCGGACGGTGCGTTCAGCGACCTCCCCGACATCACCCATGTAGTGCCCGACACCG
CGGGCGTCGCCGGCCTGCTCGGCGACAATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9286.57 Da
• Isoelectric Point: 9.0090

>AT25291 WP_019736076.1 NC_015576:606475-606726 [Mycolicibacter sinensis]
MSPLEHRLQILLDDERHRRLTAAARERGVSVASVVREAIDRGLAGPVDRRKSAGQRLLDAPDMPVPDPAELKQELDELRG
RRG

Nucleotide

• Length: 252 bp

>AT25291 NC_015576:606475-606726 [Mycolicibacter sinensis]
ATGTCTCCTTTGGAGCATCGACTGCAGATCCTGCTCGACGACGAACGCCATCGCCGACTTACCGCGGCGGCGCGGGAGCG
TGGCGTCTCGGTGGCCAGCGTGGTGCGCGAAGCCATCGATCGCGGCCTGGCAGGCCCCGTCGACCGCCGCAAATCCGCCG
GTCAGCGCCTTCTCGACGCTCCTGACATGCCGGTTCCCGACCCCGCCGAACTCAAGCAGGAATTGGACGAACTGCGCGGA
CGCCGCGGATGA

Structures

Toxin

• AlphaFold DB: F5Z2H2