Detailed information of TA system TA025290

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 594130..594743
• Replicon: chromosome
• Accession: NC_015576
• Organism: Mycobacterium sp. JDM601

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: F5Z2F7
• Locus tag: JDM601_RS02820
• Protein ID: WP_013827511.1
• Coordinates: 594339..594743 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: JDM601_RS02815
• Protein ID: WP_013827510.1
• Coordinates: 594130..594342 (+)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JDM601_RS02795	590662..591450	+	789	WP_013827506.1	class I SAM-dependent methyltransferase	-
JDM601_RS02800	591472..591948	+	477	WP_013827507.1	DUF309 domain-containing protein	-
JDM601_RS02805	591945..593030	+	1086	WP_013827508.1	galactose-1-phosphate uridylyltransferase	-
JDM601_RS02810	593027..594106	+	1080	WP_013827509.1	galactokinase	-
JDM601_RS02815	594130..594342	+	213	WP_013827510.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JDM601_RS02820	594339..594743	+	405	WP_013827511.1	PIN domain nuclease	Toxin
JDM601_RS02825	594750..595916	-	1167	WP_013827512.1	FAD-binding domain	-
JDM601_RS02830	595921..598287	-	2367	WP_041319481.1	flippase-like domain-containing protein	-
JDM601_RS02835	598644..599519	-	876	WP_013827514.1	SDR family NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (3-43)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14652.70 Da
• Isoelectric Point: 5.1895

>T25290 WP_013827511.1 NC_015576:594339-594743 [Mycolicibacter sinensis]
MILVDTSVWIEYFRATGSAAALEVRRLLSEEPDQVVTCEPIAMEILAGASNEQTYRMLERLVNGLSSLGVETATDFRSAA
GIYRAARSTGQTIRSINDCLIAAVALRHHARVAHRDADFEVIEGITGLMTTSLR

Nucleotide

• Length: 405 bp

>T25290 NC_015576:594339-594743 [Mycolicibacter sinensis]
ATGATTCTGGTCGACACCTCGGTATGGATCGAATACTTCCGGGCAACCGGGTCGGCGGCGGCGCTGGAAGTGCGGCGGCT
GCTCTCCGAAGAACCCGATCAAGTAGTGACCTGCGAGCCGATCGCGATGGAGATCTTGGCTGGCGCAAGCAACGAGCAGA
CCTACCGAATGCTTGAGCGGCTGGTCAACGGGTTGTCATCGCTGGGGGTCGAGACGGCCACGGATTTTCGGTCGGCCGCG
GGCATCTACCGTGCTGCCAGAAGCACCGGCCAGACGATTCGCAGCATCAACGACTGCCTTATCGCCGCGGTCGCGCTGCG
CCATCATGCCCGGGTTGCGCACCGCGACGCCGACTTCGAGGTCATCGAGGGGATAACCGGCCTGATGACCACATCGCTGC
GCTGA

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 7682.79 Da
• Isoelectric Point: 4.4013

>AT25290 WP_013827510.1 NC_015576:594130-594342 [Mycolicibacter sinensis]
MARTNIEIDDELTAEVMRRFGVTTKKAAVDLALRRLVGAPLTREFLLGLEGIGWAGDLDELRSEQPGELG

Nucleotide

• Length: 213 bp

>AT25290 NC_015576:594130-594342 [Mycolicibacter sinensis]
ATGGCACGCACCAATATCGAAATCGATGACGAACTCACCGCTGAGGTGATGCGTCGCTTCGGCGTGACCACCAAGAAGGC
GGCTGTTGACCTCGCGTTACGCCGTCTCGTCGGCGCGCCCTTGACCCGGGAGTTTCTGCTGGGCCTCGAGGGCATCGGGT
GGGCGGGTGACCTCGATGAGTTGCGCAGCGAACAACCCGGCGAACTCGGATGA

Structures

Toxin

• AlphaFold DB: A0A7I7JL80