Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/upstrm_HI1419-dnstrm_HI1420
Location 4573507..4574098 Replicon chromosome
Accession NZ_CP101910
Organism Pseudomonas putida strain ATCC 12633

Toxin (Protein)


Gene name PumA Uniprot ID R9VGK1
Locus tag NP430_RS20615 Protein ID WP_016488542.1
Coordinates 4573507..4573809 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID A0A1X0Z573
Locus tag NP430_RS20620 Protein ID WP_016498445.1
Coordinates 4573802..4574098 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NP430_RS20590 (NP430_20590) 4569351..4569479 - 129 WP_009683677.1 PA1414 family protein -
NP430_RS20595 (NP430_20595) 4569604..4570497 - 894 WP_016498450.1 LysR family transcriptional regulator -
NP430_RS20600 (NP430_20600) 4570604..4571773 + 1170 WP_016498449.1 MFS transporter -
NP430_RS20605 (NP430_20605) 4571903..4572829 - 927 WP_016498448.1 DMT family transporter -
NP430_RS20610 (NP430_20610) 4572936..4573331 - 396 WP_016498447.1 hypothetical protein -
NP430_RS20615 (NP430_20615) 4573507..4573809 + 303 WP_016488542.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NP430_RS20620 (NP430_20620) 4573802..4574098 + 297 WP_016498445.1 putative addiction module antidote protein Antitoxin
NP430_RS20625 (NP430_20625) 4574185..4574457 + 273 WP_016498444.1 hypothetical protein -
NP430_RS20630 (NP430_20630) 4574575..4575936 - 1362 WP_016498443.1 DEAD/DEAH box helicase -
NP430_RS20635 (NP430_20635) 4576040..4577341 + 1302 WP_016498442.1 mechanosensitive ion channel family protein -
NP430_RS20640 (NP430_20640) 4577458..4578393 - 936 WP_016498441.1 AEC family transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 4565584..4584822 19238


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(5-95)

Antitoxin

Uncharacterized protein HI1420

(8-93)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11379.12 Da        Isoelectric Point: 10.8638

>T252886 WP_016488542.1 NZ_CP101910:4573507-4573809 [Pseudomonas putida]
MKKIESSSFRHWVTGLRDLSARARIISRINRLMEGLPGDVSPVGHGVSELKIHYGPGYRVYFHQTGSTFVILLCGGDKSS
QKRDIKVAHQILRSWRMQND

Download         Length: 303 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 10687.19 Da        Isoelectric Point: 5.1023

>AT252886 WP_016498445.1 NZ_CP101910:4573802-4574098 [Pseudomonas putida]
MTETFSEFDPAAYLTTPEAIAQFMTDALETGDASYVAKAVGVVARAKGMSELAKETGLSREQLYRSFSEHGNPTLKSFLI
VMKALEIDMTARPHLARG

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB R9VGK1

Antitoxin

Source ID Structure
AlphaFold DB A0A1X0Z573

References