Detailed information of TA system TA025288

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 27029..27813
• Replicon: chromosome
• Accession: NC_015576
• Organism: Mycobacterium sp. JDM601

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: F5YWC3
• Locus tag: JDM601_RS00130
• Protein ID: WP_013826968.1
• Coordinates: 27029..27538 (-)
• Length: 170 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: JDM601_RS00135
• Protein ID: WP_085975685.1
• Coordinates: 27535..27813 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JDM601_RS00105	22276..23742	-	1467	WP_041316626.1	protein phosphatase 2C domain-containing protein	-
JDM601_RS00110	23739..24206	-	468	WP_013826964.1	FHA domain-containing protein	-
JDM601_RS00115	24358..25920	-	1563	WP_013826965.1	DUF3662 domain-containing protein	-
JDM601_RS00125	26442..26786	-	345	Protein_21	hypothetical protein	-
JDM601_RS00130	27029..27538	-	510	WP_013826968.1	GNAT family N-acetyltransferase	Toxin
JDM601_RS00135	27535..27813	-	279	WP_085975685.1	DUF1778 domain-containing protein	Antitoxin
JDM601_RS00140	28092..30230	-	2139	WP_013826970.1	topoisomerase IV	-
JDM601_RS00145	30252..32285	-	2034	WP_013826971.1	DNA gyrase subunit B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (43-145)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-85)

Sequences

Toxin

Protein

• Length: 170 a.a.
• Molecular weight: 18242.90 Da
• Isoelectric Point: 7.3847

>T25288 WP_013826968.1 NC_015576:c27538-27029 [Mycolicibacter sinensis]
VSGYSAPRPISEGDAVAEFDSGEPALDEYLRGRALANHVEGASRCFVTCRDGRVVGFYALASAAVERAGTPGRVRRNMPD
PVPVILLSRLAIDRKEQGKGLGAQLLRDAITRAVAAAEIIGVRALLVHALHEQARAFYAHFDFEPSPTDPLHLLLLIKDA
RALLDPPQK

Nucleotide

• Length: 510 bp

>T25288 NC_015576:c27538-27029 [Mycolicibacter sinensis]
GTGAGCGGTTACAGCGCGCCCCGGCCGATCAGCGAGGGCGATGCGGTCGCCGAGTTCGACAGCGGCGAGCCCGCCCTGGA
CGAGTACCTGCGGGGGCGCGCCCTGGCCAACCACGTCGAAGGTGCCTCCCGGTGCTTTGTGACGTGCCGGGACGGCCGCG
TCGTCGGGTTCTACGCGCTGGCCTCGGCGGCGGTGGAGCGCGCCGGCACACCCGGGCGGGTGCGGCGCAACATGCCCGAT
CCGGTGCCGGTGATCCTGTTGTCGCGGCTGGCGATCGACCGCAAGGAACAGGGCAAGGGGCTGGGCGCGCAGCTGCTGCG
CGACGCGATTACTCGCGCGGTGGCCGCTGCGGAGATCATCGGTGTGCGGGCACTGCTCGTGCACGCCCTGCACGAGCAGG
CGCGGGCGTTCTACGCGCACTTCGATTTCGAGCCCTCACCGACCGACCCGCTGCACCTGCTGCTCCTGATCAAAGACGCC
CGCGCCCTGCTCGACCCGCCGCAGAAGTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10443.92 Da
• Isoelectric Point: 4.5098

>AT25288 WP_085975685.1 NC_015576:c27813-27535 [Mycolicibacter sinensis]
MVDMAMKTERFAVRLTAEQDALIRRAAEVEGTDLTNFTVTATLAHAREVLADRRLFMLDASAWSEFVSVLDRPVSQQPRL
EKLFAEPSIFDE

Nucleotide

• Length: 279 bp

>AT25288 NC_015576:c27813-27535 [Mycolicibacter sinensis]
ATGGTTGACATGGCTATGAAGACGGAGCGGTTCGCGGTGCGCCTGACTGCTGAGCAGGACGCGCTGATTCGTCGTGCCGC
CGAAGTGGAGGGGACCGATCTGACCAACTTCACAGTGACTGCGACGCTGGCGCATGCCCGCGAGGTGCTCGCCGATCGGC
GGCTGTTCATGCTTGATGCGTCGGCCTGGTCGGAGTTCGTGTCGGTGCTGGACCGCCCGGTGTCGCAGCAGCCGCGGCTG
GAGAAGTTGTTCGCGGAGCCTTCGATCTTCGACGAGTGA

Structures

Toxin

• AlphaFold DB: F5YWC3