Detailed information of TA system TA025286

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 1932651..1933284
• Replicon: chromosome
• Accession: NC_015572
• Organism: Methylomonas methanica MC09

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0A2J6
• Locus tag: METME_RS08850
• Protein ID: WP_013818429.1
• Coordinates: 1932871..1933284 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0A2J5
• Locus tag: METME_RS08845
• Protein ID: WP_013818428.1
• Coordinates: 1932651..1932884 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
METME_RS08830	1928288..1929190	+	903	WP_013818425.1	hypothetical protein	-
METME_RS08835	1929198..1931516	-	2319	WP_013818426.1	carbamoyltransferase HypF	-
METME_RS08840	1931516..1932559	-	1044	WP_013818427.1	hydrogenase expression/formation protein HypE	-
METME_RS08845	1932651..1932884	+	234	WP_013818428.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
METME_RS08850	1932871..1933284	+	414	WP_013818429.1	PIN domain-containing protein	Toxin
METME_RS08855	1933259..1934395	-	1137	WP_013818430.1	hydrogenase formation protein HypD	-
METME_RS08860	1934392..1934625	-	234	WP_013818431.1	HypC/HybG/HupF family hydrogenase formation chaperone	-
METME_RS08870	1935005..1935562	-	558	WP_148261964.1	hypothetical protein	-
METME_RS08875	1935585..1935920	-	336	WP_041363950.1	hypothetical protein	-
METME_RS24630	1936085..1936339	-	255	WP_041363952.1	hypothetical protein	-
METME_RS08885	1936336..1936533	-	198	WP_013818433.1	hypothetical protein	-
METME_RS08890	1936530..1936967	-	438	WP_013818434.1	hypothetical protein	-
METME_RS08895	1936988..1937815	-	828	WP_013818435.1	hydrogenase nickel incorporation protein HypB	-
METME_RS08900	1937815..1938156	-	342	WP_013818436.1	hydrogenase maturation nickel metallochaperone HypA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-116)

Antitoxin

• SpoVT-AbrB domain (5-39)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15049.28 Da
• Isoelectric Point: 4.9049

>T25286 WP_013818429.1 NC_015572:1932871-1933284 [Methylomonas methanica MC09]
MKTAKPFFDTNVLLYLLSADHRKADRAEILLSAGGTISVQVLNEFASVASRKLRMPYPEIRETLQIVKAVCATQALSLET
HEQGLVVAERYGFSLYDSMIISAALLAGSKVLFSEDMQHGQEIEGGLTIINPFSETD

Nucleotide

• Length: 414 bp

>T25286 NC_015572:1932871-1933284 [Methylomonas methanica MC09]
ATGAAAACCGCTAAACCTTTCTTCGATACCAATGTTTTACTGTATTTATTGTCAGCCGATCACCGTAAAGCCGATAGAGC
CGAAATCTTGCTGAGTGCCGGCGGCACTATAAGCGTGCAAGTGCTAAACGAGTTTGCCTCGGTCGCGTCGCGAAAGCTGC
GTATGCCGTACCCGGAAATTCGTGAAACGCTACAAATCGTCAAAGCCGTCTGCGCAACACAAGCGCTAAGTCTGGAAACC
CACGAGCAAGGTTTGGTTGTTGCGGAGCGTTACGGATTTTCGTTGTACGACAGCATGATTATCAGCGCGGCACTGCTTGC
AGGCAGTAAAGTGTTGTTTTCCGAAGATATGCAACATGGGCAGGAAATTGAAGGCGGTTTGACCATTATTAATCCGTTTT
CCGAGACTGATTAG

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8773.04 Da
• Isoelectric Point: 8.6582

>AT25286 WP_013818428.1 NC_015572:1932651-1932884 [Methylomonas methanica MC09]
MQVAKWGNSLAVRLPAAVVEALQLKEGDDIEIHVAGERSFAVDKKADNLELLSRLRKYRGRLPADFKFDRLEAHENR

Nucleotide

• Length: 234 bp

>AT25286 NC_015572:1932651-1932884 [Methylomonas methanica MC09]
ATGCAAGTAGCCAAATGGGGTAATAGTTTAGCGGTAAGATTACCCGCCGCTGTCGTCGAAGCATTGCAGTTGAAGGAAGG
GGACGATATCGAGATTCACGTCGCCGGCGAACGCAGCTTTGCTGTCGATAAAAAAGCCGATAATCTGGAATTGCTGAGCA
GATTACGTAAGTATCGCGGTCGATTACCGGCGGATTTTAAGTTCGATCGCTTGGAAGCGCATGAAAACCGCTAA

Structures

Toxin

• AlphaFold DB: G0A2J6

Antitoxin

• AlphaFold DB: G0A2J5