Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 546102..546739 Replicon chromosome
Accession NZ_CP101904
Organism Bacillus velezensis strain Ba-0321

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag NPS25_RS02655 Protein ID WP_003156187.1
Coordinates 546389..546739 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag NPS25_RS02650 Protein ID WP_003156188.1
Coordinates 546102..546383 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NPS25_RS02630 (NPS25_02630) 542467..543066 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
NPS25_RS02635 (NPS25_02635) 543159..543524 + 366 WP_007410229.1 holo-ACP synthase -
NPS25_RS02640 (NPS25_02640) 543689..544696 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
NPS25_RS02645 (NPS25_02645) 544813..545982 + 1170 WP_039252648.1 alanine racemase -
NPS25_RS02650 (NPS25_02650) 546102..546383 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
NPS25_RS02655 (NPS25_02655) 546389..546739 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
NPS25_RS02660 (NPS25_02660) 546857..547678 + 822 WP_003156182.1 STAS domain-containing protein -
NPS25_RS02665 (NPS25_02665) 547683..548048 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
NPS25_RS02670 (NPS25_02670) 548051..548452 + 402 WP_003156178.1 anti-sigma regulatory factor -
NPS25_RS02675 (NPS25_02675) 548464..549471 + 1008 WP_015416875.1 PP2C family protein-serine/threonine phosphatase -
NPS25_RS02680 (NPS25_02680) 549535..549864 + 330 WP_033575044.1 anti-sigma factor antagonist -
NPS25_RS02685 (NPS25_02685) 549861..550343 + 483 WP_007609591.1 anti-sigma B factor RsbW -
NPS25_RS02690 (NPS25_02690) 550309..551097 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
NPS25_RS02695 (NPS25_02695) 551097..551699 + 603 WP_007410234.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T252859 WP_003156187.1 NZ_CP101904:546389-546739 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT252859 WP_003156188.1 NZ_CP101904:546102-546383 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References