Detailed information of TA system TA025285

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 229235..229845
• Replicon: chromosome
• Accession: NC_015572
• Organism: Methylomonas methanica MC09

Toxin (Protein)

• Gene name: relE
• Uniprot ID: F9ZZK3
• Locus tag: METME_RS01020
• Protein ID: WP_013816935.1
• Coordinates: 229591..229845 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: METME_RS01015
• Protein ID: WP_013816934.1
• Coordinates: 229235..229594 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
METME_RS01000	225705..227738	-	2034	WP_013816931.1	DNA sulfur modification protein DndD	-
METME_RS01005	227735..227953	-	219	WP_013816932.1	hypothetical protein	-
METME_RS01010	227926..229065	-	1140	WP_013816933.1	DNA phosphorothioation system sulfurtransferase DndC	-
METME_RS01015	229235..229594	-	360	WP_013816934.1	helix-turn-helix domain-containing protein	Antitoxin
METME_RS01020	229591..229845	-	255	WP_013816935.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
METME_RS01025	229934..231364	-	1431	WP_013816936.1	bifunctional D-glycero-beta-D-manno-heptose-7-phosphate kinase/D-glycero-beta-D-manno-heptose 1-phosphate adenylyltransferase HldE	-
METME_RS01030	231437..231784	+	348	WP_013816937.1	arsenate reductase (glutaredoxin)	-
METME_RS01035	231784..232380	+	597	WP_013816938.1	NAD(P)H:quinone oxidoreductase	-
METME_RS01040	232386..233039	-	654	WP_013816939.1	FkbM family methyltransferase	-
METME_RS01045	233085..233909	-	825	WP_041363682.1	alpha-1,2-fucosyltransferase	-
METME_RS23145	233932..234732	-	801	WP_013816941.1	glycosyltransferase family 2 protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1836..263813	261977

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-83)

Antitoxin

• Cro/C1-type helix-turn-helix domain (62-118)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9746.47 Da
• Isoelectric Point: 10.0955

>T25285 WP_013816935.1 NC_015572:c229845-229591 [Methylomonas methanica MC09]
MYRVIYRKTAAKALLKMPHRAAEQFKEAFEQMAINDEDGLDIKKLEGREGFRLRIGGYRAIYLKLNDVLIIEVLKVGSRG
DIYK

Nucleotide

• Length: 255 bp

>T25285 NC_015572:c229845-229591 [Methylomonas methanica MC09]
ATGTACCGAGTCATCTATCGAAAAACGGCGGCCAAAGCATTGCTGAAAATGCCGCATAGAGCAGCCGAACAATTCAAAGA
AGCATTCGAGCAGATGGCAATAAATGACGAAGACGGACTGGACATAAAAAAGCTGGAAGGCCGAGAAGGTTTTAGATTAC
GGATTGGCGGTTACCGTGCGATTTACCTCAAATTGAACGATGTGCTGATTATAGAAGTACTTAAGGTTGGTAGCCGGGGA
GATATTTACAAATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13154.02 Da
• Isoelectric Point: 4.4057

>AT25285 WP_013816934.1 NC_015572:c229594-229235 [Methylomonas methanica MC09]
MSVQFIEQNGQKQYAVMPVADYEKLLEKAEMLGDITAYDQALTSEDELVPAEIVNRLLNGENKIKVWREHRGLTQAGLAE
SCKMAQASIAQMESGKRTGTIAALKKIAETLDVDLDDLV

Nucleotide

• Length: 360 bp

>AT25285 NC_015572:c229594-229235 [Methylomonas methanica MC09]
ATGAGCGTGCAATTTATCGAACAAAACGGCCAAAAACAATATGCGGTTATGCCGGTAGCCGACTACGAAAAGCTGTTGGA
AAAAGCCGAAATGCTGGGCGACATTACTGCCTATGACCAAGCCCTGACCAGCGAAGACGAGCTGGTGCCTGCCGAAATCG
TAAATCGGCTGCTGAATGGCGAGAATAAAATCAAAGTATGGCGCGAGCATCGCGGTCTGACTCAGGCAGGGCTGGCAGAA
AGCTGCAAAATGGCTCAAGCCAGCATCGCACAAATGGAAAGCGGCAAGCGAACCGGAACCATTGCCGCACTTAAAAAAAT
AGCCGAAACGTTGGATGTGGATTTGGATGATTTGGTTTAG

Structures

Toxin

• AlphaFold DB: F9ZZK3