Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3910533..3911109 Replicon chromosome
Accession NZ_CP101866
Organism Escherichia coli strain LP5-1

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag NPX81_RS19080 Protein ID WP_001297643.1
Coordinates 3910822..3911109 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag NPX81_RS19075 Protein ID WP_000063156.1
Coordinates 3910533..3910835 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NPX81_RS19060 (3907129) 3907129..3909279 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
NPX81_RS19065 (3909329) 3909329..3909532 + 204 WP_000467859.1 YbdD/YjiX family protein -
NPX81_RS19070 (3909543) 3909543..3910499 + 957 WP_001393094.1 GTPase -
NPX81_RS19075 (3910533) 3910533..3910835 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
NPX81_RS19080 (3910822) 3910822..3911109 - 288 WP_001297643.1 BrnT family toxin Toxin
NPX81_RS19090 (3912331) 3912331..3913562 + 1232 Protein_3733 multidrug efflux MFS transporter MdtM -
NPX81_RS19095 (3913603) 3913603..3914883 + 1281 WP_016230805.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3911308..3911811 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T252821 WP_001297643.1 NZ_CP101866:c3911109-3910822 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT252821 WP_000063156.1 NZ_CP101866:c3910835-3910533 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References