252817

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 3534801..3535480 Replicon chromosome
Accession NZ_CP101866
Organism Escherichia coli strain LP5-1

Toxin (Protein)


Gene name ykfI Uniprot ID S1FHS4
Locus tag NPX81_RS17120 Protein ID WP_000854680.1
Coordinates 3535139..3535480 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID S1EWR7
Locus tag NPX81_RS17115 Protein ID WP_000070396.1
Coordinates 3534801..3535118 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NPX81_RS17070 (3530239) 3530239..3531060 + 822 WP_230069315.1 DUF932 domain-containing protein -
NPX81_RS17075 (3531277) 3531277..3531978 + 702 WP_001581462.1 WYL domain-containing protein -
NPX81_RS17080 (3532019) 3532019..3532255 + 237 WP_001144031.1 protein YpjK -
NPX81_RS17085 (3532255) 3532255..3532698 + 444 WP_032199444.1 lipoprotein YafY -
NPX81_RS17090 (3532721) 3532721..3533188 + 468 WP_032200803.1 protein YkfB -
NPX81_RS17095 (3533265) 3533265..3533504 + 240 WP_180513156.1 DUF905 domain-containing protein -
NPX81_RS17100 (3533602) 3533602..3534060 + 459 WP_032177818.1 antirestriction protein -
NPX81_RS17105 (3534076) 3534076..3534552 + 477 WP_072667534.1 RadC family protein -
NPX81_RS17110 (3534561) 3534561..3534782 + 222 WP_000691994.1 DUF987 domain-containing protein -
NPX81_RS17115 (3534801) 3534801..3535118 + 318 WP_000070396.1 type IV toxin-antitoxin system antitoxin YafW Antitoxin
NPX81_RS17120 (3535139) 3535139..3535480 + 342 WP_000854680.1 type IV toxin-antitoxin system toxin YkfI Toxin
NPX81_RS17125 (3536024) 3536024..3536224 + 201 Protein_3351 integrase -
NPX81_RS17130 (3536291) 3536291..3537453 + 1163 WP_085947771.1 IS3-like element IS3 family transposase -
NPX81_RS17135 (3537483) 3537483..3538562 + 1080 Protein_3353 tyrosine-type recombinase/integrase -
NPX81_RS17140 (3538643) 3538643..3539353 - 711 WP_230069332.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3533602..3607111 73509


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(6-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12892.95 Da        Isoelectric Point: 9.6543

>T252817 WP_000854680.1 NZ_CP101866:3535139-3535480 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11982.62 Da        Isoelectric Point: 7.0270

>AT252817 WP_000070396.1 NZ_CP101866:3534801-3535118 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHS4

Antitoxin

Source ID Structure
AlphaFold DB S1EWR7

References