Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 4348960..4349797 Replicon chromosome
Accession NZ_CP101858
Organism Escherichia coli strain LP50-1

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag NPX80_RS21005 Protein ID WP_000227784.1
Coordinates 4348960..4349502 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag NPX80_RS21010 Protein ID WP_001297137.1
Coordinates 4349486..4349797 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NPX80_RS20985 (4344499) 4344499..4345410 - 912 WP_000705847.1 2-dehydropantoate 2-reductase -
NPX80_RS20990 (4345578) 4345578..4346069 + 492 WP_001138904.1 nucleotide binding protein YajQ -
NPX80_RS20995 (4346197) 4346197..4347561 - 1365 WP_001000978.1 MFS transporter -
NPX80_RS21000 (4347969) 4347969..4348904 + 936 WP_001297127.1 tetratricopeptide repeat protein -
NPX80_RS21005 (4348960) 4348960..4349502 - 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
NPX80_RS21010 (4349486) 4349486..4349797 - 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
NPX80_RS21015 (4349982) 4349982..4350872 - 891 WP_000971336.1 heme o synthase -
NPX80_RS21020 (4350884) 4350884..4351213 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
NPX80_RS21025 (4351213) 4351213..4351827 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
NPX80_RS21030 (4351817) 4351817..4353808 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
NPX80_RS21035 (4353830) 4353830..4354777 - 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T252792 WP_000227784.1 NZ_CP101858:c4349502-4348960 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT252792 WP_001297137.1 NZ_CP101858:c4349797-4349486 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References