Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 2728290..2729090 Replicon chromosome
Accession NZ_CP101858
Organism Escherichia coli strain LP50-1

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag NPX80_RS13345 Protein ID WP_000342449.1
Coordinates 2728290..2728817 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag NPX80_RS13350 Protein ID WP_001277108.1
Coordinates 2728824..2729090 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NPX80_RS13320 (2723365) 2723365..2724132 - 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
NPX80_RS13325 (2724129) 2724129..2725406 - 1278 WP_000803771.1 branched chain amino acid ABC transporter permease LivM -
NPX80_RS13330 (2725403) 2725403..2726329 - 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
NPX80_RS13335 (2726377) 2726377..2727486 - 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
NPX80_RS13340 (2727910) 2727910..2728293 + 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -
NPX80_RS13345 (2728290) 2728290..2728817 - 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
NPX80_RS13350 (2728824) 2728824..2729090 - 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
NPX80_RS13355 (2729240) 2729240..2730343 - 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
NPX80_RS13360 (2730614) 2730614..2731468 - 855 WP_063100092.1 RNA polymerase sigma factor RpoH -
NPX80_RS13365 (2731713) 2731713..2732771 - 1059 WP_001042003.1 permease-like cell division protein FtsX -
NPX80_RS13370 (2732764) 2732764..2733432 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T252786 WP_000342449.1 NZ_CP101858:c2728817-2728290 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT252786 WP_001277108.1 NZ_CP101858:c2729090-2728824 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References