Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 2015472..2016055 Replicon chromosome
Accession NZ_CP101858
Organism Escherichia coli strain LP50-1

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag NPX80_RS09835 Protein ID WP_000254745.1
Coordinates 2015472..2015807 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag NPX80_RS09840 Protein ID WP_000581937.1
Coordinates 2015807..2016055 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NPX80_RS09815 (2010941) 2010941..2011303 + 363 WP_000034929.1 hypothetical protein -
NPX80_RS09820 (2011359) 2011359..2012657 - 1299 WP_000036723.1 phosphopyruvate hydratase -
NPX80_RS09825 (2012745) 2012745..2014382 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
NPX80_RS09830 (2014610) 2014610..2015401 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
NPX80_RS09835 (2015472) 2015472..2015807 - 336 WP_000254745.1 endoribonuclease MazF Toxin
NPX80_RS09840 (2015807) 2015807..2016055 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
NPX80_RS09845 (2016133) 2016133..2018367 - 2235 WP_000226815.1 GTP pyrophosphokinase -
NPX80_RS09850 (2018415) 2018415..2019716 - 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2000010..2016055 16045


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T252783 WP_000254745.1 NZ_CP101858:c2015807-2015472 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT252783 WP_000581937.1 NZ_CP101858:c2016055-2015807 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References