Detailed information of TA system TA252768

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 4013003..4014477
• Replicon: chromosome
• Accession: NZ_CP101847
• Organism: Xanthomonas oryzae pv. oryzae strain YNCX

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: NO935_RS18605
• Protein ID: WP_258526804.1
• Coordinates: 4013003..4013410 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: NO935_RS18610
• Protein ID: WP_075244497.1
• Coordinates: 4013407..4014477 (+)
• Length: 357 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NO935_RS18575 (NO935_18575)	4008609..4009280	+	672	WP_011407759.1	class I SAM-dependent methyltransferase	-
NO935_RS18585 (NO935_18585)	4009582..4010373	-	792	WP_171970779.1	polysaccharide deacetylase family protein	-
NO935_RS18590 (NO935_18590)	4010419..4011345	+	927	WP_011257932.1	Grx4 family monothiol glutaredoxin	-
NO935_RS18595 (NO935_18595)	4011677..4011898	+	222	WP_103057334.1	DarT ssDNA thymidine ADP-ribosyltransferase family protein	-
NO935_RS18600 (NO935_18600)	4011908..4012870	-	963	WP_011409560.1	IS1595-like element ISXo2 family transposase	-
NO935_RS18605 (NO935_18605)	4013003..4013410	+	408	WP_258526804.1	DUF4433 domain-containing protein	Toxin
NO935_RS18610 (NO935_18610)	4013407..4014477	+	1071	WP_075244497.1	macro domain-containing protein	Antitoxin
NO935_RS18615 (NO935_18615)	4014515..4015750	-	1236	WP_012445794.1	ISL3-like element ISXoo13 family transposase	-
NO935_RS18620 (NO935_18620)	4016052..4016378	+	327	Protein_3570	hypothetical protein	-
NO935_RS18625 (NO935_18625)	4016739..4018340	+	1602	WP_011407751.1	rhamnogalacturonan lyase B N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	4011908..4015750	3842

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (1-134)

Antitoxin

• Macro domain (18-130)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 16045.22 Da
• Isoelectric Point: 7.5501

>T252768 WP_258526804.1 NZ_CP101847:4013003-4013410 [Xanthomonas oryzae pv. oryzae]
MLHNIHTGWRGIPRRPNEDIVILVSSLHHVAERRWPFLFTDSHASCQLASFYSDLSDLDKIDWPLLQARDFRRDPDDPAK
FERYQAEALIHRHLPLDGLRGIVCHTDGLKQTIERQLQERNLKLPVHTRTDWYFR

Nucleotide

• Length: 408 bp

Antitoxin

Protein

• Length: 357 a.a.
• Molecular weight: 39942.02 Da
• Isoelectric Point: 6.0080

>AT252768 WP_075244497.1 NZ_CP101847:4013407-4014477 [Xanthomonas oryzae pv. oryzae]
MITFTQGNLLESGAEALVNTVNTVGVMGKGIALMFKERFKENFLRYAAACKDNQVRIGKIFVTEVNELDGPRWIINFPTK
QHWRGDSRIEWITEGLQDLHLFLIENKVKSIAIPPLGAGNGGLDWAEVRPLIEEVLAGLDTDILVFEPTLKYQNVAKRSG
VEKLTPARALIAELVRRYWVLGMECGLLEIQKLAWFLERNIERAGLPELDLRFSAHKYGPYADRLRHLLNGLDGSYLHCD
KRISDADPLDVIWFDDNRKSFVQTYLKSEAKQYIPALEATAALIDGFESPFGMELLATVDWLIVREGISAQVADVREGIR
HWPGGQEAAARKDRLFDDRALGIALKRLASGAIAVA

Nucleotide

• Length: 1071 bp