Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5267717..5268342 Replicon chromosome
Accession NZ_CP101835
Organism Klebsiella pneumoniae strain GN4549

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag NPH93_RS25875 Protein ID WP_002882817.1
Coordinates 5267717..5268100 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag NPH93_RS25880 Protein ID WP_004150355.1
Coordinates 5268100..5268342 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NPH93_RS25860 (NPH93_25855) 5265083..5265985 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
NPH93_RS25865 (NPH93_25860) 5265982..5266617 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
NPH93_RS25870 (NPH93_25865) 5266614..5267543 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
NPH93_RS25875 (NPH93_25870) 5267717..5268100 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
NPH93_RS25880 (NPH93_25875) 5268100..5268342 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
NPH93_RS25885 (NPH93_25880) 5268547..5269464 + 918 WP_002882812.1 alpha/beta hydrolase -
NPH93_RS25890 (NPH93_25885) 5269478..5270419 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
NPH93_RS25895 (NPH93_25890) 5270464..5270901 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
NPH93_RS25900 (NPH93_25895) 5270898..5271758 - 861 WP_002882807.1 virulence factor BrkB family protein -
NPH93_RS25905 (NPH93_25900) 5271752..5272351 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T252756 WP_002882817.1 NZ_CP101835:c5268100-5267717 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT252756 WP_004150355.1 NZ_CP101835:c5268342-5268100 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References