Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4685389..4686199 Replicon chromosome
Accession NZ_CP101835
Organism Klebsiella pneumoniae strain GN4549

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag NPH93_RS23085 Protein ID WP_002887280.1
Coordinates 4685389..4685922 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag NPH93_RS23090 Protein ID WP_002887278.1
Coordinates 4685933..4686199 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NPH93_RS23080 (NPH93_23075) 4684220..4685341 + 1122 WP_002887282.1 cupin domain-containing protein -
NPH93_RS23085 (NPH93_23080) 4685389..4685922 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
NPH93_RS23090 (NPH93_23085) 4685933..4686199 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
NPH93_RS23095 (NPH93_23090) 4686302..4687735 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
NPH93_RS23100 (NPH93_23095) 4687725..4688408 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
NPH93_RS23105 (NPH93_23100) 4688580..4689965 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
NPH93_RS23110 (NPH93_23105) 4689983..4690327 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T252753 WP_002887280.1 NZ_CP101835:c4685922-4685389 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT252753 WP_002887278.1 NZ_CP101835:c4686199-4685933 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References