Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 519944..520580 Replicon chromosome
Accession NZ_CP101833
Organism Bacillus pumilus strain NDY-10

Toxin (Protein)


Gene name mazF Uniprot ID K2MHT4
Locus tag NPA43_RS02535 Protein ID WP_003214169.1
Coordinates 520230..520580 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID W8QJ31
Locus tag NPA43_RS02530 Protein ID WP_003214273.1
Coordinates 519944..520225 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NPA43_RS02510 (NPA43_02510) 516089..516694 - 606 WP_099728420.1 rhomboid family intramembrane serine protease -
NPA43_RS02515 (NPA43_02515) 516789..517154 + 366 WP_256499323.1 holo-ACP synthase -
NPA43_RS02520 (NPA43_02520) 517315..518331 + 1017 WP_099728418.1 outer membrane lipoprotein carrier protein LolA -
NPA43_RS02525 (NPA43_02525) 518471..519652 + 1182 WP_099728417.1 alanine racemase -
NPA43_RS02530 (NPA43_02530) 519944..520225 + 282 WP_003214273.1 hypothetical protein Antitoxin
NPA43_RS02535 (NPA43_02535) 520230..520580 + 351 WP_003214169.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
NPA43_RS02540 (NPA43_02540) 520698..521528 + 831 WP_099728416.1 RsbT co-antagonist protein RsbRA -
NPA43_RS02545 (NPA43_02545) 521533..521901 + 369 WP_003214235.1 RsbT antagonist protein RsbS -
NPA43_RS02550 (NPA43_02550) 521904..522305 + 402 WP_256499324.1 anti-sigma regulatory factor -
NPA43_RS02555 (NPA43_02555) 522316..523323 + 1008 WP_230031323.1 PP2C family protein-serine/threonine phosphatase -
NPA43_RS02560 (NPA43_02560) 523383..523712 + 330 WP_099728413.1 anti-sigma factor antagonist -
NPA43_RS02565 (NPA43_02565) 523709..524197 + 489 WP_230031324.1 anti-sigma B factor RsbW -
NPA43_RS02570 (NPA43_02570) 524163..524951 + 789 WP_099728411.1 RNA polymerase sigma factor SigB -
NPA43_RS02575 (NPA43_02575) 524951..525550 + 600 WP_099728410.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12976.99 Da        Isoelectric Point: 5.1663

>T252741 WP_003214169.1 NZ_CP101833:520230-520580 [Bacillus pumilus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEINAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQVSLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10864.25 Da        Isoelectric Point: 5.2726

>AT252741 WP_003214273.1 NZ_CP101833:519944-520225 [Bacillus pumilus]
MSESSATNEMKIRLPEALMSEIERVAEQDKVTRNELIYRATKTYIRERNIRQNRESMRRGYMEMAKINLNISSEAQYAEF
EAENTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K2MHT4

Antitoxin

Source ID Structure
AlphaFold DB A0A081L854

References