Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2711684..2712335 Replicon chromosome
Accession NZ_CP101831
Organism Lacticaseibacillus paracasei strain SMN-LBK

Toxin (Protein)


Gene name pemK Uniprot ID K6S8L5
Locus tag NPC72_RS13085 Protein ID WP_003567661.1
Coordinates 2711684..2712067 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID K6RKI5
Locus tag NPC72_RS13090 Protein ID WP_003567665.1
Coordinates 2712087..2712335 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NPC72_RS13080 (NPC72_13080) 2710979..2711500 - 522 WP_003567657.1 QueT transporter family protein -
NPC72_RS13085 (NPC72_13085) 2711684..2712067 - 384 WP_003567661.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
NPC72_RS13090 (NPC72_13090) 2712087..2712335 - 249 WP_003567665.1 antitoxin Antitoxin
NPC72_RS13095 (NPC72_13095) 2712403..2713539 - 1137 WP_003581082.1 alanine racemase -
NPC72_RS13100 (NPC72_13100) 2713526..2713900 - 375 WP_003567669.1 holo-ACP synthase -
NPC72_RS13105 (NPC72_13105) 2714070..2715578 - 1509 WP_003567670.1 DEAD/DEAH box helicase -
NPC72_RS13110 (NPC72_13110) 2715704..2715847 - 144 WP_003588978.1 hypothetical protein -
NPC72_RS13115 (NPC72_13115) 2715878..2717266 - 1389 WP_003567677.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13773.03 Da        Isoelectric Point: 10.9764

>T252740 WP_003567661.1 NZ_CP101831:c2712067-2711684 [Lacticaseibacillus paracasei]
MDVSVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSTKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8938.08 Da        Isoelectric Point: 4.4610

>AT252740 WP_003567665.1 NZ_CP101831:c2712335-2712087 [Lacticaseibacillus paracasei]
MKKQTSVAVTFDDAIKARLDAYCELNDIDAQAVITEAVATFLKSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K6S8L5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BNY2

References