Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5183200..5183825 Replicon chromosome
Accession NZ_CP101790
Organism Klebsiella pneumoniae strain hvKP859

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag NPJ86_RS25220 Protein ID WP_002882817.1
Coordinates 5183200..5183583 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag NPJ86_RS25225 Protein ID WP_004150355.1
Coordinates 5183583..5183825 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NPJ86_RS25205 (NPJ86_25205) 5180566..5181468 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
NPJ86_RS25210 (NPJ86_25210) 5181465..5182100 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
NPJ86_RS25215 (NPJ86_25215) 5182097..5183026 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
NPJ86_RS25220 (NPJ86_25220) 5183200..5183583 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
NPJ86_RS25225 (NPJ86_25225) 5183583..5183825 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
NPJ86_RS25230 (NPJ86_25230) 5184030..5184947 + 918 WP_021466676.1 alpha/beta hydrolase -
NPJ86_RS25235 (NPJ86_25235) 5184961..5185902 - 942 WP_073558103.1 fatty acid biosynthesis protein FabY -
NPJ86_RS25240 (NPJ86_25240) 5185947..5186384 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
NPJ86_RS25245 (NPJ86_25245) 5186381..5187241 - 861 WP_004146232.1 virulence factor BrkB family protein -
NPJ86_RS25250 (NPJ86_25250) 5187235..5187834 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T252731 WP_002882817.1 NZ_CP101790:c5183583-5183200 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT252731 WP_004150355.1 NZ_CP101790:c5183825-5183583 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References