Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5360372..5360997 Replicon chromosome
Accession NZ_CP101776
Organism Klebsiella pneumoniae strain hvKP340

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag NPJ84_RS26455 Protein ID WP_002882817.1
Coordinates 5360372..5360755 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag NPJ84_RS26460 Protein ID WP_004150355.1
Coordinates 5360755..5360997 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NPJ84_RS26440 (NPJ84_26440) 5357738..5358640 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
NPJ84_RS26445 (NPJ84_26445) 5358637..5359272 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
NPJ84_RS26450 (NPJ84_26450) 5359269..5360198 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
NPJ84_RS26455 (NPJ84_26455) 5360372..5360755 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
NPJ84_RS26460 (NPJ84_26460) 5360755..5360997 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
NPJ84_RS26465 (NPJ84_26465) 5361202..5362119 + 918 WP_002882812.1 alpha/beta hydrolase -
NPJ84_RS26470 (NPJ84_26470) 5362133..5363074 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
NPJ84_RS26475 (NPJ84_26475) 5363119..5363556 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
NPJ84_RS26480 (NPJ84_26480) 5363553..5364413 - 861 WP_002882807.1 virulence factor BrkB family protein -
NPJ84_RS26485 (NPJ84_26485) 5364407..5365006 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T252680 WP_002882817.1 NZ_CP101776:c5360755-5360372 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T252680 NZ_CP101776:c5360755-5360372 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT252680 WP_004150355.1 NZ_CP101776:c5360997-5360755 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT252680 NZ_CP101776:c5360997-5360755 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References