Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5325485..5326110 Replicon chromosome
Accession NZ_CP101764
Organism Klebsiella pneumoniae strain hvKP319

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag NPJ83_RS26195 Protein ID WP_002882817.1
Coordinates 5325485..5325868 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag NPJ83_RS26200 Protein ID WP_004150355.1
Coordinates 5325868..5326110 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NPJ83_RS26180 (NPJ83_26180) 5322851..5323753 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
NPJ83_RS26185 (NPJ83_26185) 5323750..5324385 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
NPJ83_RS26190 (NPJ83_26190) 5324382..5325311 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
NPJ83_RS26195 (NPJ83_26195) 5325485..5325868 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
NPJ83_RS26200 (NPJ83_26200) 5325868..5326110 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
NPJ83_RS26205 (NPJ83_26205) 5326315..5327232 + 918 WP_002882812.1 alpha/beta hydrolase -
NPJ83_RS26210 (NPJ83_26210) 5327246..5328187 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
NPJ83_RS26215 (NPJ83_26215) 5328232..5328669 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
NPJ83_RS26220 (NPJ83_26220) 5328666..5329526 - 861 WP_002882807.1 virulence factor BrkB family protein -
NPJ83_RS26225 (NPJ83_26225) 5329520..5330119 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T252623 WP_002882817.1 NZ_CP101764:c5325868-5325485 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT252623 WP_004150355.1 NZ_CP101764:c5326110-5325868 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References