Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4739451..4740261 Replicon chromosome
Accession NZ_CP101764
Organism Klebsiella pneumoniae strain hvKP319

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag NPJ83_RS23375 Protein ID WP_002887280.1
Coordinates 4739451..4739984 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag NPJ83_RS23380 Protein ID WP_002887278.1
Coordinates 4739995..4740261 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NPJ83_RS23370 (NPJ83_23370) 4738282..4739403 + 1122 WP_002887282.1 cupin domain-containing protein -
NPJ83_RS23375 (NPJ83_23375) 4739451..4739984 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
NPJ83_RS23380 (NPJ83_23380) 4739995..4740261 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
NPJ83_RS23385 (NPJ83_23385) 4740364..4741797 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
NPJ83_RS23390 (NPJ83_23390) 4741787..4742470 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
NPJ83_RS23395 (NPJ83_23395) 4742642..4744027 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
NPJ83_RS23400 (NPJ83_23400) 4744045..4744389 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T252620 WP_002887280.1 NZ_CP101764:c4739984-4739451 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT252620 WP_002887278.1 NZ_CP101764:c4740261-4739995 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References