Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 140641..141166 Replicon plasmid pVir_150040X1B1
Accession NZ_CP101728
Organism Klebsiella pneumoniae strain 150040X1B1

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag NPN21_RS28630 Protein ID WP_001159868.1
Coordinates 140641..140946 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag NPN21_RS28635 Protein ID WP_000813634.1
Coordinates 140948..141166 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NPN21_RS28615 (136573) 136573..137739 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
NPN21_RS28620 (138327) 138327..139082 - 756 WP_000852146.1 replication initiation protein RepE -
NPN21_RS28625 (139834) 139834..140640 - 807 WP_000016982.1 site-specific integrase -
NPN21_RS28630 (140641) 140641..140946 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
NPN21_RS28635 (140948) 140948..141166 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
NPN21_RS28640 (141758) 141758..142246 + 489 WP_011254646.1 hypothetical protein -
NPN21_RS28645 (142280) 142280..143413 - 1134 WP_000545984.1 DUF3800 domain-containing protein -
NPN21_RS28650 (143580) 143580..144353 - 774 WP_000905949.1 hypothetical protein -
NPN21_RS28655 (144366) 144366..144866 - 501 WP_000528932.1 HEPN family nuclease -
NPN21_RS28660 (145131) 145131..145361 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin -
NPN21_RS28665 (145358) 145358..145774 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / sitABCD iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA 1..172348 172348


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T252578 WP_001159868.1 NZ_CP101728:c140946-140641 [Klebsiella pneumoniae]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT252578 WP_000813634.1 NZ_CP101728:c141166-140948 [Klebsiella pneumoniae]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References