Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5381728..5382353 Replicon chromosome
Accession NZ_CP101726
Organism Klebsiella pneumoniae strain 150040X1B1

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag NPN21_RS26535 Protein ID WP_002882817.1
Coordinates 5381728..5382111 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag NPN21_RS26540 Protein ID WP_004150355.1
Coordinates 5382111..5382353 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NPN21_RS26520 (NPN21_26520) 5379094..5379996 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
NPN21_RS26525 (NPN21_26525) 5379993..5380628 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
NPN21_RS26530 (NPN21_26530) 5380625..5381554 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
NPN21_RS26535 (NPN21_26535) 5381728..5382111 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
NPN21_RS26540 (NPN21_26540) 5382111..5382353 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
NPN21_RS26545 (NPN21_26545) 5382558..5383475 + 918 WP_002882812.1 alpha/beta hydrolase -
NPN21_RS26550 (NPN21_26550) 5383489..5384430 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
NPN21_RS26555 (NPN21_26555) 5384475..5384912 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
NPN21_RS26560 (NPN21_26560) 5384909..5385769 - 861 WP_002882807.1 virulence factor BrkB family protein -
NPN21_RS26565 (NPN21_26565) 5385763..5386362 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T252565 WP_002882817.1 NZ_CP101726:c5382111-5381728 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT252565 WP_004150355.1 NZ_CP101726:c5382353-5382111 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References