Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 518381..519017 Replicon chromosome
Accession NZ_CP101718
Organism Bacillus halotolerans strain S-5

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag NPA28_RS02655 Protein ID WP_003156187.1
Coordinates 518667..519017 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag NPA28_RS02650 Protein ID WP_003225183.1
Coordinates 518381..518662 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NPA28_RS02630 (NPA28_02630) 514738..515337 - 600 WP_044161737.1 rhomboid family intramembrane serine protease -
NPA28_RS02635 (NPA28_02635) 515432..515797 + 366 WP_256766654.1 holo-ACP synthase -
NPA28_RS02640 (NPA28_02640) 515963..516979 + 1017 WP_256766957.1 outer membrane lipoprotein carrier protein LolA -
NPA28_RS02645 (NPA28_02645) 517096..518265 + 1170 WP_044161733.1 alanine racemase -
NPA28_RS02650 (NPA28_02650) 518381..518662 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
NPA28_RS02655 (NPA28_02655) 518667..519017 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
NPA28_RS02660 (NPA28_02660) 519133..519957 + 825 WP_082687807.1 RsbT co-antagonist protein RsbRA -
NPA28_RS02665 (NPA28_02665) 519962..520327 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
NPA28_RS02670 (NPA28_02670) 520331..520732 + 402 WP_024714447.1 serine/threonine-protein kinase RsbT -
NPA28_RS02675 (NPA28_02675) 520744..521751 + 1008 WP_044161721.1 phosphoserine phosphatase RsbU -
NPA28_RS02680 (NPA28_02680) 521812..522141 + 330 WP_024120301.1 anti-sigma factor antagonist RsbV -
NPA28_RS02685 (NPA28_02685) 522138..522620 + 483 WP_024120302.1 anti-sigma B factor RsbW -
NPA28_RS02690 (NPA28_02690) 522586..523374 + 789 WP_024120303.1 RNA polymerase sigma factor SigB -
NPA28_RS02695 (NPA28_02695) 523374..523973 + 600 WP_217828106.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T252549 WP_003156187.1 NZ_CP101718:518667-519017 [Bacillus halotolerans]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT252549 WP_003225183.1 NZ_CP101718:518381-518662 [Bacillus halotolerans]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References