Detailed information of TA system TA025249

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 555059..556608
• Replicon: chromosome
• Accession: NC_015566
• Organism: Serratia sp. AS12

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A7U3YYG4
• Locus tag: SERAS12_RS02590
• Protein ID: WP_013811338.1
• Coordinates: 555310..556608 (+)
• Length: 433 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: SERAS12_RS02585
• Protein ID: WP_004953056.1
• Coordinates: 555059..555307 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SERAS12_RS02555	550274..551344	+	1071	WP_013811333.1	LPS export ABC transporter permease LptG	-
SERAS12_RS02565	551838..552053	+	216	WP_013811334.1	hypothetical protein	-
SERAS12_RS02570	552086..552787	-	702	WP_013811335.1	hypothetical protein	-
SERAS12_RS02575	553127..553762	+	636	WP_013811336.1	carbonic anhydrase	-
SERAS12_RS02580	554192..554650	-	459	WP_013811337.1	hypothetical protein	-
SERAS12_RS02585	555059..555307	+	249	WP_004953056.1	helix-turn-helix domain-containing protein	Antitoxin
SERAS12_RS02590	555310..556608	+	1299	WP_013811338.1	type II toxin-antitoxin system HipA family toxin	Toxin
SERAS12_RS02595	557293..559929	+	2637	WP_013811339.1	Z1 domain-containing protein	-
SERAS12_RS25270	559935..560933	+	999	WP_013811340.1	PD-(D/E)XK motif protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-399)

Antitoxin

• Cro/C1-type helix-turn-helix domain (10-64)

Sequences

Toxin

Protein

• Length: 433 a.a.
• Molecular weight: 48216.28 Da
• Isoelectric Point: 5.6236

>T25249 WP_013811338.1 NC_015566:555310-556608 [Serratia sp. AS12]
MEKLRVALNGTVVGTLEKHAGGAMSFVYHEEWLAQAGARAISLSLPLQPEPFRGDVVYNFFDNLLPDSELIRSRLQARFK
TATKQPFDLLSSIGSDCVGAIQLYPEHADVAPVKQVNAVPVSDEEIEQLLLGYREAPLGMEAGIDDFRISLAGAQEKTAL
LWFGNQWCRPQGSTPTSHIIKLPIGHIAHNNLDLSESCENEWLCLRIAEQFGLPVAQAELAHFGEQKVLIVERFDRRWAR
DNSWLIRLPQEDFCQALGIAHALKYEADGGPGIAAAMELLLGSQQPLLDRDIFFKSQIVFWMLAAIDGHAKNFSLFIEQE
TAYRMTPLYDVLSGYPFMNARGIAPQKAKMAMAVLGQSRHYRWSQILPRHFISTARAVGYSEEKTLQVMAELKAQTEQAI
ANVEAMLPDEFPRLVSDAIFAGVRRQAERLVG

Nucleotide

• Length: 1299 bp

>T25249 NC_015566:555310-556608 [Serratia sp. AS12]
ATGGAAAAGCTACGTGTGGCGCTGAATGGCACTGTTGTTGGGACGCTGGAAAAGCACGCGGGCGGCGCGATGTCGTTTGT
TTACCACGAAGAGTGGCTGGCACAAGCCGGTGCGCGCGCCATATCGTTATCCCTGCCGCTACAACCAGAACCGTTTCGCG
GTGACGTGGTGTATAACTTTTTCGACAACCTGCTGCCGGACAGCGAACTTATTCGCAGCCGCCTACAGGCGCGTTTTAAA
ACGGCAACCAAACAGCCTTTTGATCTGCTTTCCAGCATTGGCAGCGACTGCGTGGGTGCGATCCAGCTTTACCCTGAACA
TGCCGATGTGGCCCCGGTCAAGCAAGTGAACGCCGTGCCTGTCAGTGACGAAGAAATAGAGCAACTTCTGCTGGGGTACC
GCGAGGCACCGCTGGGCATGGAGGCGGGTATTGATGATTTCCGGATCTCTCTGGCGGGTGCGCAGGAAAAGACCGCGCTG
CTTTGGTTCGGCAATCAGTGGTGTCGGCCACAGGGGAGTACGCCAACCAGCCACATCATAAAACTGCCCATTGGGCATAT
TGCACATAACAATTTGGATCTCAGTGAAAGCTGTGAGAACGAGTGGTTATGTTTGCGCATTGCAGAACAGTTTGGCCTGC
CGGTAGCGCAGGCTGAGTTGGCGCATTTTGGCGAGCAGAAGGTCCTGATCGTTGAGCGTTTTGACCGGCGTTGGGCGCGG
GATAACAGTTGGCTGATTCGTTTGCCGCAGGAGGATTTTTGCCAGGCGTTAGGCATTGCTCATGCGCTGAAATATGAGGC
TGATGGTGGGCCCGGTATTGCGGCTGCTATGGAGTTGCTATTGGGCTCTCAACAACCCTTGCTCGATCGGGACATTTTCT
TTAAAAGCCAAATCGTATTCTGGATGCTGGCGGCCATTGATGGGCATGCCAAAAACTTTAGCCTGTTCATTGAGCAGGAA
ACGGCCTATCGAATGACTCCGCTTTACGATGTGCTCTCGGGTTACCCATTCATGAACGCCCGTGGCATCGCGCCGCAGAA
AGCAAAAATGGCCATGGCCGTGCTGGGGCAAAGTCGCCATTACCGTTGGAGCCAGATCTTGCCTCGGCACTTTATCTCTA
CCGCGAGAGCGGTGGGGTATTCCGAAGAGAAAACGCTGCAGGTGATGGCTGAGCTAAAAGCGCAAACGGAGCAGGCGATC
GCTAATGTGGAAGCGATGCTGCCGGATGAGTTTCCTCGTCTCGTGAGTGATGCGATTTTTGCTGGGGTTCGGCGGCAGGC
TGAGAGGTTGGTGGGGTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9263.60 Da
• Isoelectric Point: 10.4887

>AT25249 WP_004953056.1 NC_015566:555059-555307 [Serratia sp. AS12]
MKITSAKMLAHALRNERKKRNQSQSKTADSIGLKQATVSAFENNPDGTRLETLFKLLAALDLELQVTPRGKPVDPKTWDQ
EW

Nucleotide

• Length: 249 bp

>AT25249 NC_015566:555059-555307 [Serratia sp. AS12]
ATGAAAATCACCTCGGCAAAGATGTTGGCGCATGCGCTACGCAATGAGCGTAAAAAGCGTAACCAGAGCCAAAGCAAAAC
CGCTGACAGCATCGGATTAAAACAGGCCACGGTTTCCGCCTTTGAAAACAACCCGGACGGCACCCGGCTGGAGACGTTGT
TTAAACTGCTTGCTGCGCTGGATTTAGAGCTACAAGTGACTCCGCGCGGTAAGCCGGTCGATCCCAAAACCTGGGATCAG
GAGTGGTGA

Structures

Toxin

• AlphaFold DB: A0A7U3YYG4