Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 94594..95237 Replicon plasmid pM171-1.1
Accession NZ_CP101667
Organism Escherichia coli strain M171-1

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag NO473_RS22760 Protein ID WP_001044768.1
Coordinates 94594..95010 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag NO473_RS22765 Protein ID WP_001261287.1
Coordinates 95007..95237 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NO473_RS22745 (89898) 89898..90791 - 894 WP_001553853.1 S-4TM family putative pore-forming effector -
NO473_RS22750 (90834) 90834..91829 - 996 WP_000246636.1 hypothetical protein -
NO473_RS22755 (92294) 92294..94432 + 2139 WP_039002805.1 AAA family ATPase -
NO473_RS22760 (94594) 94594..95010 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
NO473_RS22765 (95007) 95007..95237 - 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
NO473_RS22770 (95543) 95543..96091 + 549 WP_039002808.1 hypothetical protein -
NO473_RS22775 (96143) 96143..96973 + 831 WP_223201786.1 hypothetical protein -
NO473_RS22780 (97024) 97024..98661 + 1638 WP_223201785.1 hypothetical protein -
NO473_RS22785 (98924) 98924..100057 + 1134 Protein_113 DUF3800 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / sul3 / aph(3')-Ia / sul2 / floR / dfrA12 / aadA2 / cmlA1 / ant(3'')-Ia / tet(M) - 1..122198 122198


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T252482 WP_001044768.1 NZ_CP101667:c95010-94594 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT252482 WP_001261287.1 NZ_CP101667:c95237-95007 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References