Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3809694..3810348 Replicon chromosome
Accession NZ_CP101666
Organism Escherichia coli strain M171-1

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag NO473_RS18550 Protein ID WP_000244777.1
Coordinates 3809941..3810348 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag NO473_RS18545 Protein ID WP_000354046.1
Coordinates 3809694..3809960 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NO473_RS18520 (3804863) 3804863..3805606 + 744 WP_000951964.1 SDR family oxidoreductase -
NO473_RS18525 (3805663) 3805663..3807096 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
NO473_RS18530 (3807141) 3807141..3807452 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
NO473_RS18535 (3807616) 3807616..3808275 + 660 WP_000250274.1 hemolysin III family protein -
NO473_RS18540 (3808471) 3808471..3809451 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
NO473_RS18545 (3809694) 3809694..3809960 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
NO473_RS18550 (3809941) 3809941..3810348 + 408 WP_000244777.1 protein YgfX Toxin
NO473_RS18555 (3810388) 3810388..3810909 - 522 WP_001055874.1 flavodoxin FldB -
NO473_RS18560 (3811021) 3811021..3811917 + 897 WP_072643464.1 site-specific tyrosine recombinase XerD -
NO473_RS18565 (3811942) 3811942..3812652 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
NO473_RS18570 (3812658) 3812658..3814391 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T252473 WP_000244777.1 NZ_CP101666:3809941-3810348 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT252473 WP_000354046.1 NZ_CP101666:3809694-3809960 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References