Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2478667..2479164 Replicon chromosome
Accession NZ_CP101666
Organism Escherichia coli strain M171-1

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag NO473_RS12150 Protein ID WP_001173343.1
Coordinates 2478667..2478840 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag NO473_RS12155 Protein ID WP_000398619.1
Coordinates 2478868..2479164 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NO473_RS12140 (2475337) 2475337..2476794 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
NO473_RS12145 (2477031) 2477031..2478548 + 1518 WP_001295074.1 lysine--tRNA ligase -
NO473_RS12150 (2478667) 2478667..2478840 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
NO473_RS12155 (2478868) 2478868..2479164 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
NO473_RS12160 (2479391) 2479391..2479663 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
NO473_RS12165 (2479675) 2479675..2479905 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
NO473_RS12170 (2480086) 2480086..2481717 + 1632 WP_001216477.1 sensor histidine kinase -
NO473_RS12175 (2481714) 2481714..2482433 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2455622..2479164 23542


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T252468 WP_001173343.1 NZ_CP101666:c2478840-2478667 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT252468 WP_000398619.1 NZ_CP101666:c2479164-2478868 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References