Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 2260019..2260595 Replicon chromosome
Accession NZ_CP101666
Organism Escherichia coli strain M171-1

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag NO473_RS11040 Protein ID WP_001297643.1
Coordinates 2260308..2260595 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag NO473_RS11035 Protein ID WP_000063156.1
Coordinates 2260019..2260321 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NO473_RS11020 (2256615) 2256615..2258765 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
NO473_RS11025 (2258815) 2258815..2259018 + 204 WP_000467859.1 YbdD/YjiX family protein -
NO473_RS11030 (2259029) 2259029..2259985 + 957 WP_001297640.1 GTPase -
NO473_RS11035 (2260019) 2260019..2260321 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
NO473_RS11040 (2260308) 2260308..2260595 - 288 WP_001297643.1 BrnT family toxin Toxin
NO473_RS11045 (2260794) 2260794..2261491 - 698 Protein_2155 IS1 family transposase -
NO473_RS11050 (2261548) 2261548..2261643 + 96 Protein_2156 dienelactone hydrolase family protein -
NO473_RS11055 (2261789) 2261789..2263021 + 1233 WP_024186283.1 multidrug efflux MFS transporter MdtM -
NO473_RS11060 (2263062) 2263062..2264342 + 1281 WP_001037400.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T252465 WP_001297643.1 NZ_CP101666:c2260595-2260308 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT252465 WP_000063156.1 NZ_CP101666:c2260321-2260019 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References