Detailed information of TA system TA025246

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-yefM/YoeB-YefM
• Location: 4413424..4413940
• Replicon: chromosome
• Accession: NC_015564
• Organism: Amycolicicoccus subflavus DQS3-9A1

Toxin (Protein)

• Gene name: relB
• Uniprot ID: F6EL48
• Locus tag: AS9A_RS20470
• Protein ID: WP_013809060.1
• Coordinates: 4413683..4413940 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: F6EL47
• Locus tag: AS9A_RS20465
• Protein ID: WP_013809059.1
• Coordinates: 4413424..4413681 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AS9A_RS20450	4408618..4409415	+	798	WP_041452308.1	hypothetical protein	-
AS9A_RS20455	4409408..4410520	+	1113	WP_013809057.1	XRE family transcriptional regulator	-
AS9A_RS20460	4410588..4413338	+	2751	WP_013809058.1	AAA family ATPase	-
AS9A_RS20465	4413424..4413681	+	258	WP_013809059.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
AS9A_RS20470	4413683..4413940	+	258	WP_013809060.1	Txe/YoeB family addiction module toxin	Toxin
AS9A_RS23460	4414085..4414195	+	111	Protein_4096	Lsr2 family protein	-
AS9A_RS20475	4414196..4415401	-	1206	WP_013798052.1	IS30 family transposase	-
AS9A_RS20480	4415367..4415684	+	318	WP_148262520.1	Lsr2 family protein	-
AS9A_RS20485	4416088..4416726	-	639	WP_013809063.1	TetR/AcrR family transcriptional regulator	-
AS9A_RS20490	4417034..4417558	+	525	WP_013809064.1	PaaI family thioesterase	-
AS9A_RS20495	4417711..4418712	-	1002	WP_013809066.1	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4373673..4452553	78880

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10389.76 Da
• Isoelectric Point: 8.0063

>T25246 WP_013809060.1 NC_015564:4413683-4413940 [Hoyosella subflava DQS3-9A1]
MLLVWDDNAWQDYLWWQQQDRKILKRINTLLQDITRNGNEGIGKPEPLKYDFAGYWSRRITDEHRLVYKVTETEVRIATC
RYHYG

Nucleotide

• Length: 258 bp

>T25246 NC_015564:4413683-4413940 [Hoyosella subflava DQS3-9A1]
ATGCTGCTGGTCTGGGACGACAACGCCTGGCAGGACTACCTCTGGTGGCAACAGCAGGACCGCAAGATTCTCAAAAGGAT
CAACACCCTCCTGCAGGACATCACACGCAACGGCAATGAAGGAATCGGCAAACCCGAACCCCTCAAATACGACTTCGCCG
GATACTGGTCCAGACGGATCACCGACGAACACCGCCTCGTCTACAAAGTCACCGAAACCGAAGTCCGTATCGCCACCTGC
CGCTACCACTACGGATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9648.82 Da
• Isoelectric Point: 4.6899

>AT25246 WP_013809059.1 NC_015564:4413424-4413681 [Hoyosella subflava DQS3-9A1]
MKTMSYTESRARYAEVLDGVTNDREEVVITRAGHEPVVMVSLDDYESLRETAHLMRSPANARRLLDAMERLEAGRGQPRD
LLDAD

Nucleotide

• Length: 258 bp

>AT25246 NC_015564:4413424-4413681 [Hoyosella subflava DQS3-9A1]
ATGAAGACTATGAGCTACACCGAGTCCCGGGCGCGCTACGCCGAGGTTCTCGACGGGGTCACCAATGACCGCGAGGAGGT
CGTCATCACCCGTGCTGGCCACGAGCCGGTGGTCATGGTCTCCCTCGACGACTACGAGTCCCTGCGCGAGACCGCCCACC
TGATGCGATCCCCGGCCAACGCTCGCCGACTCCTGGACGCGATGGAAAGGCTCGAGGCCGGCCGCGGACAGCCCCGCGAC
CTGCTCGACGCCGACTGA

Structures

Toxin

• AlphaFold DB: F6EL48

Antitoxin

• AlphaFold DB: F6EL47