Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 466452..467089 Replicon chromosome
Accession NZ_CP101661
Organism Bacillus amyloliquefaciens strain B408

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag NO220_RS02405 Protein ID WP_003156187.1
Coordinates 466739..467089 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag NO220_RS02400 Protein ID WP_003156188.1
Coordinates 466452..466733 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NO220_RS02380 (NO220_02380) 462817..463416 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
NO220_RS02385 (NO220_02385) 463509..463874 + 366 WP_014304402.1 holo-ACP synthase -
NO220_RS02390 (NO220_02390) 464039..465046 + 1008 WP_003156191.1 outer membrane lipoprotein carrier protein LolA -
NO220_RS02395 (NO220_02395) 465163..466332 + 1170 WP_003156189.1 alanine racemase -
NO220_RS02400 (NO220_02400) 466452..466733 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
NO220_RS02405 (NO220_02405) 466739..467089 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
NO220_RS02410 (NO220_02410) 467207..468028 + 822 WP_003156182.1 STAS domain-containing protein -
NO220_RS02415 (NO220_02415) 468033..468398 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
NO220_RS02420 (NO220_02420) 468401..468802 + 402 WP_003156178.1 anti-sigma regulatory factor -
NO220_RS02425 (NO220_02425) 468814..469821 + 1008 WP_003156177.1 PP2C family protein-serine/threonine phosphatase -
NO220_RS02430 (NO220_02430) 469885..470214 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
NO220_RS02435 (NO220_02435) 470211..470693 + 483 WP_003156173.1 anti-sigma B factor RsbW -
NO220_RS02440 (NO220_02440) 470659..471447 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
NO220_RS02445 (NO220_02445) 471447..472049 + 603 WP_003156170.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T252454 WP_003156187.1 NZ_CP101661:466739-467089 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT252454 WP_003156188.1 NZ_CP101661:466452-466733 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References