Detailed information of TA system TA025245

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2531876..2532438
• Replicon: chromosome
• Accession: NC_015564
• Organism: Amycolicicoccus subflavus DQS3-9A1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: AS9A_RS11920
• Protein ID: WP_013807271.1
• Coordinates: 2532118..2532438 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: AS9A_RS11915
• Protein ID: WP_041451050.1
• Coordinates: 2531876..2532097 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AS9A_RS11895	2526995..2528485	-	1491	WP_013807266.1	aldehyde dehydrogenase family protein	-
AS9A_RS11900	2528504..2529181	+	678	WP_083826533.1	TetR family transcriptional regulator	-
AS9A_RS11905	2529275..2530735	+	1461	WP_041451049.1	glyceraldehyde-3-phosphate dehydrogenase	-
AS9A_RS11910	2530772..2531836	-	1065	WP_013807269.1	alpha/beta hydrolase	-
AS9A_RS11915	2531876..2532097	+	222	WP_041451050.1	hypothetical protein	Antitoxin
AS9A_RS11920	2532118..2532438	+	321	WP_013807271.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AS9A_RS11925	2532574..2533782	+	1209	WP_013807272.1	DNA polymerase IV	-
AS9A_RS11930	2533787..2534929	-	1143	WP_013807273.1	NAD(P)/FAD-dependent oxidoreductase	-
AS9A_RS11935	2535057..2535968	+	912	WP_013807274.1	cation transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 11635.24 Da
• Isoelectric Point: 10.4287

>T25245 WP_013807271.1 NC_015564:2532118-2532438 [Hoyosella subflava DQS3-9A1]
VDFSTPGGAKDLTRSAIIVSNDRANRAAERLERGLVTVVPISGDVRRFDSFHVLIPAGDAGFPRDFKAHCDQVRSISVTR
VGERTGTVPKRVLSEIERALRVHLDL

Nucleotide

• Length: 321 bp

>T25245 NC_015564:2532118-2532438 [Hoyosella subflava DQS3-9A1]
GTGGACTTCAGTACGCCAGGTGGCGCGAAGGACCTGACCAGGTCAGCCATCATCGTGAGCAATGACCGGGCCAACCGCGC
CGCGGAAAGGCTGGAGCGGGGGCTCGTGACAGTCGTGCCCATCTCAGGGGACGTACGGCGTTTCGATTCGTTCCACGTGC
TGATTCCGGCCGGGGACGCGGGGTTCCCGCGGGACTTCAAGGCTCACTGTGACCAAGTCCGATCCATTTCGGTGACACGG
GTCGGAGAGCGCACGGGCACGGTCCCAAAGCGGGTGCTCAGCGAGATCGAACGGGCACTAAGGGTCCACCTCGACCTCTG
A

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8266.25 Da
• Isoelectric Point: 4.5600

>AT25245 WP_041451050.1 NC_015564:2531876-2532097 [Hoyosella subflava DQS3-9A1]
MKFLVTLPREDVRTLDDYVRRTGLESRNDGLRAAIQALRDNRLEDAYRVALESWSDASDAAVWELAIADGLSK

Nucleotide

• Length: 222 bp

>AT25245 NC_015564:2531876-2532097 [Hoyosella subflava DQS3-9A1]
ATGAAGTTTCTCGTGACGCTCCCTCGGGAGGATGTCAGAACCCTTGATGATTATGTGCGCCGAACTGGGCTGGAATCGCG
GAACGACGGCTTGCGTGCTGCGATCCAGGCACTGCGCGATAACCGGCTCGAGGACGCGTACCGAGTTGCGCTGGAGAGTT
GGTCGGACGCCAGCGACGCAGCCGTATGGGAACTGGCGATCGCGGATGGACTGTCAAAGTGA