Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2535440..2536091 Replicon chromosome
Accession NZ_CP101643
Organism Lacticaseibacillus rhamnosus strain CLK 101

Toxin (Protein)


Gene name pemK Uniprot ID K8Q4Y0
Locus tag NOS86_RS11670 Protein ID WP_005686631.1
Coordinates 2535440..2535823 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID K8QH19
Locus tag NOS86_RS11675 Protein ID WP_005686632.1
Coordinates 2535843..2536091 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NOS86_RS11665 (NOS86_11665) 2534399..2534926 - 528 WP_005691221.1 QueT transporter family protein -
NOS86_RS11670 (NOS86_11670) 2535440..2535823 - 384 WP_005686631.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
NOS86_RS11675 (NOS86_11675) 2535843..2536091 - 249 WP_005686632.1 antitoxin Antitoxin
NOS86_RS11680 (NOS86_11680) 2536203..2537342 - 1140 WP_005691215.1 alanine racemase -
NOS86_RS11685 (NOS86_11685) 2537329..2537703 - 375 WP_005686634.1 holo-ACP synthase -
NOS86_RS11690 (NOS86_11690) 2537872..2539380 - 1509 WP_005686635.1 DEAD/DEAH box helicase -
NOS86_RS11695 (NOS86_11695) 2539654..2541042 - 1389 WP_014571607.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-115)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13757.03 Da        Isoelectric Point: 10.9764

>T252411 WP_005686631.1 NZ_CP101643:c2535823-2535440 [Lacticaseibacillus rhamnosus]
MDVTVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSAKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8936.13 Da        Isoelectric Point: 4.4778

>AT252411 WP_005686632.1 NZ_CP101643:c2536091-2535843 [Lacticaseibacillus rhamnosus]
MKKETSVTVALDSAIKARLDAYCELNDMGVQDVITEAVAKFLQSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K8Q4Y0

Antitoxin

Source ID Structure
AlphaFold DB K8QH19

References