Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 478489..479126 Replicon chromosome
Accession NZ_CP101621
Organism Bacillus velezensis strain 906

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag NN913_RS02490 Protein ID WP_003156187.1
Coordinates 478776..479126 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag NN913_RS02485 Protein ID WP_003156188.1
Coordinates 478489..478770 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NN913_RS02465 (NN913_02460) 474854..475453 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
NN913_RS02470 (NN913_02465) 475546..475911 + 366 WP_071392104.1 holo-ACP synthase -
NN913_RS02475 (NN913_02470) 476076..477083 + 1008 WP_003156191.1 outer membrane lipoprotein carrier protein LolA -
NN913_RS02480 (NN913_02475) 477200..478369 + 1170 WP_003156189.1 alanine racemase -
NN913_RS02485 (NN913_02480) 478489..478770 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
NN913_RS02490 (NN913_02485) 478776..479126 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
NN913_RS02495 (NN913_02490) 479244..480065 + 822 WP_014304404.1 STAS domain-containing protein -
NN913_RS02500 (NN913_02495) 480070..480435 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
NN913_RS02505 (NN913_02500) 480438..480839 + 402 WP_003156178.1 anti-sigma regulatory factor -
NN913_RS02510 (NN913_02505) 480851..481858 + 1008 WP_255764145.1 PP2C family protein-serine/threonine phosphatase -
NN913_RS02515 (NN913_02510) 481922..482251 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
NN913_RS02520 (NN913_02515) 482248..482730 + 483 WP_003156173.1 anti-sigma B factor RsbW -
NN913_RS02525 (NN913_02520) 482696..483484 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
NN913_RS02530 (NN913_02525) 483484..484086 + 603 WP_255764146.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T252406 WP_003156187.1 NZ_CP101621:478776-479126 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT252406 WP_003156188.1 NZ_CP101621:478489-478770 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References