Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 20789..21432 Replicon plasmid pDC71-3
Accession NZ_CP101618
Organism Escherichia coli strain DC71

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag NOF56_RS24005 Protein ID WP_001044768.1
Coordinates 20789..21205 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag NOF56_RS24010 Protein ID WP_001261287.1
Coordinates 21202..21432 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NOF56_RS23990 (16093) 16093..16986 - 894 WP_001553853.1 S-4TM family putative pore-forming effector -
NOF56_RS23995 (17029) 17029..18024 - 996 WP_000246636.1 hypothetical protein -
NOF56_RS24000 (18489) 18489..20627 + 2139 WP_000350635.1 AAA family ATPase -
NOF56_RS24005 (20789) 20789..21205 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
NOF56_RS24010 (21202) 21202..21432 - 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
NOF56_RS24015 (21739) 21739..24858 + 3120 WP_023909028.1 hypothetical protein -
NOF56_RS24020 (25121) 25121..26254 + 1134 WP_000545983.1 DUF3800 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mph(A) / aadA5 / sul1 / tet(B) - 1..72929 72929
- flank IS/Tn - - 15453..15956 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T252400 WP_001044768.1 NZ_CP101618:c21205-20789 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT252400 WP_001261287.1 NZ_CP101618:c21432-21202 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References