252395

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pmenTA/darT(toxin)
Location 2829601..2831321 Replicon chromosome
Accession NZ_CP101615
Organism Escherichia coli strain DC71

Toxin (Protein)


Gene name pmenT Uniprot ID A0A7U9J2A7
Locus tag NOF56_RS13720 Protein ID WP_000312833.1
Coordinates 2829601..2830257 (+) Length 219 a.a.

Antitoxin (Protein)


Gene name pmenA Uniprot ID B7UP19
Locus tag NOF56_RS13725 Protein ID WP_000634203.1
Coordinates 2830254..2831321 (+) Length 356 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NOF56_RS13705 (2825516) 2825516..2825755 + 240 WP_024224214.1 hypothetical protein -
NOF56_RS13710 (2825795) 2825795..2828233 + 2439 WP_032180025.1 type I restriction-modification system subunit M -
NOF56_RS13715 (2828233) 2828233..2829588 + 1356 WP_223349791.1 restriction endonuclease subunit S -
NOF56_RS13720 (2829601) 2829601..2830257 + 657 WP_000312833.1 toxin-antitoxin system toxin DNA ADP-ribosyl transferase DarT Toxin
NOF56_RS13725 (2830254) 2830254..2831321 + 1068 WP_000634203.1 toxin-antitoxin system antitoxin DNA ADP-ribosyl glycohydrolase DarG Antitoxin
NOF56_RS13730 (2831340) 2831340..2834435 + 3096 WP_000108736.1 HsdR family type I site-specific deoxyribonuclease -
NOF56_RS13735 (2834435) 2834435..2835151 + 717 WP_001122107.1 SprT family zinc-dependent metalloprotease -
NOF56_RS13740 (2835496) 2835496..2836110 + 615 Protein_2692 IS3-like element IS600 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage blaCMY-2 - 2822163..2852436 30273
- inside Prophage blaCMY-2 - 2812729..2857418 44689


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ssDNA thymidine ADP-ribosyltransferase, DarT

(17-217)

Antitoxin

Macro domain

(18-129)


Sequences

Toxin        


Download         Length: 219 a.a.        Molecular weight: 25233.56 Da        Isoelectric Point: 6.2145

>T252395 WP_000312833.1 NZ_CP101615:2829601-2830257 [Escherichia coli]
MAYDYSASLNPQKALIWRIVHRDNIPWILDNGLHCGNSLVQAENWINIGNPELIGKRAGHPVPVGTGGTLHDYVPFYFTP
FSPMLMNIHSGRGGIKQRPNEEIVILVSNLRNVAAHDVPFVFTDSHAYYNWTNYYTSLDSLDQIDWPILQARDFRRDPDD
PAKFERYQAEALIWQHCPISLLDGIICYSEEVRLQLEQWLFQRNLTMSVHTRAGWYFS

Download         Length: 657 bp

Antitoxin


Download         Length: 356 a.a.        Molecular weight: 40672.70 Da        Isoelectric Point: 5.6927

>AT252395 WP_000634203.1 NZ_CP101615:2830254-2831321 [Escherichia coli]
MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPENMKVYALACKQKQVITGKMFITETGELMGPRWIVNFPTK
QHWRADSRMEWIEDGLQDLRRFLIEENVQSIAIPPLGAGNGGLNWPDVRAQIESALGDLQDVDILIYQPTEKYQNVAKST
GVKKLTPARAAIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQDDILKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDQKKEHVNAYLNNEAREWLPALEQVSQLIDGFESPFGLELLATVDWLLSRGECQPTLDSVKEG
LHQWPAGERWASRKLRLFDNNNLQFAINRVMEFHC

Download         Length: 1068 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9J2A7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9J2B9

References