Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 477083..477720 Replicon chromosome
Accession NZ_CP101610
Organism Bacillus siamensis strain WYJ-E14

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag NNG64_RS02440 Protein ID WP_003156187.1
Coordinates 477370..477720 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag NNG64_RS02435 Protein ID WP_003156188.1
Coordinates 477083..477364 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NNG64_RS02415 (473449) 473449..474048 - 600 WP_016937174.1 rhomboid family intramembrane serine protease -
NNG64_RS02420 (474141) 474141..474506 + 366 WP_060963545.1 holo-ACP synthase -
NNG64_RS02425 (474671) 474671..475678 + 1008 WP_016937172.1 outer membrane lipoprotein carrier protein LolA -
NNG64_RS02430 (475794) 475794..476963 + 1170 WP_016937171.1 alanine racemase -
NNG64_RS02435 (477083) 477083..477364 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
NNG64_RS02440 (477370) 477370..477720 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
NNG64_RS02445 (477841) 477841..478662 + 822 WP_060963544.1 STAS domain-containing protein -
NNG64_RS02450 (478667) 478667..479032 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
NNG64_RS02455 (479035) 479035..479436 + 402 WP_003156178.1 anti-sigma regulatory factor -
NNG64_RS02460 (479448) 479448..480455 + 1008 WP_016937170.1 PP2C family protein-serine/threonine phosphatase -
NNG64_RS02465 (480519) 480519..480848 + 330 WP_016937169.1 anti-sigma factor antagonist -
NNG64_RS02470 (480845) 480845..481327 + 483 WP_003156173.1 anti-sigma B factor RsbW -
NNG64_RS02475 (481293) 481293..482081 + 789 WP_060963543.1 RNA polymerase sigma factor SigB -
NNG64_RS02480 (482081) 482081..482683 + 603 WP_060963542.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T252374 WP_003156187.1 NZ_CP101610:477370-477720 [Bacillus siamensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT252374 WP_003156188.1 NZ_CP101610:477083-477364 [Bacillus siamensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References