Detailed information of TA system TA025235

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 424856..425483
• Replicon: chromosome
• Accession: NC_015555
• Organism: Thermoanaerobacterium xylanolyticum LX-11

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: F6BH30
• Locus tag: THEXY_RS02220
• Protein ID: WP_013787226.1
• Coordinates: 425133..425483 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I3VUF9
• Locus tag: THEXY_RS02215
• Protein ID: WP_013297076.1
• Coordinates: 424856..425131 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
THEXY_RS02195	420625..420987	+	363	WP_013787222.1	hypothetical protein	-
THEXY_RS02200	421065..422591	+	1527	WP_013787223.1	bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase	-
THEXY_RS02205	422605..423588	+	984	WP_013787224.1	outer membrane lipoprotein carrier protein LolA	-
THEXY_RS02210	423602..424768	+	1167	WP_013787225.1	alanine racemase	-
THEXY_RS02215	424856..425131	+	276	WP_013297076.1	ribbon-helix-helix protein, CopG family	Antitoxin
THEXY_RS02220	425133..425483	+	351	WP_013787226.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
THEXY_RS02225	425601..426047	+	447	WP_013787227.1	hypothetical protein	-
THEXY_RS02230	426160..428181	+	2022	WP_013787228.1	hypothetical protein	-
THEXY_RS02235	428219..429298	+	1080	WP_013787229.1	polysaccharide pyruvyl transferase CsaB	-
THEXY_RS02240	429301..430032	+	732	WP_013787230.1	WecB/TagA/CpsF family glycosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-43)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12916.98 Da
• Isoelectric Point: 6.4704

>T25235 WP_013787226.1 NC_015555:425133-425483 [Thermoanaerobacterium xylanolyticum LX-11]
MIVKRGDILYADLSPVIGSEQGGVRPVLVIQNDIGNKYSPTVIVSAITSQINKAKLPTHVEINGTEYGLNKDSVILLEQI
RTIDKKRLREKIGHFDQDMMEKVNEALQISLGLIDF

Nucleotide

• Length: 351 bp

>T25235 NC_015555:425133-425483 [Thermoanaerobacterium xylanolyticum LX-11]
ATGATTGTAAAGCGTGGAGATATTTTATATGCTGATTTAAGCCCTGTCATAGGCTCAGAACAGGGTGGTGTTAGGCCCGT
ACTTGTAATACAGAACGATATCGGCAATAAGTACAGCCCTACAGTTATCGTGTCAGCAATAACATCTCAGATAAACAAGG
CTAAATTGCCGACACATGTTGAGATAAATGGAACGGAATATGGTCTTAATAAAGATTCGGTTATTTTATTAGAGCAAATA
AGGACTATAGATAAAAAGCGGCTAAGGGAAAAGATAGGCCATTTTGACCAAGATATGATGGAAAAAGTAAATGAGGCATT
GCAGATAAGCCTTGGATTGATAGATTTTTAA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10514.31 Da
• Isoelectric Point: 7.7813

>AT25235 WP_013297076.1 NC_015555:424856-425131 [Thermoanaerobacterium xylanolyticum LX-11]
VGETKRILVSLPESLLEEVDVLAAMENRNRSEFIREAMKLYIKERKKMKIRESMKKGYLEMAAINAELAELGLTADNECF
NGYEKKLKKCD

Nucleotide

• Length: 276 bp

>AT25235 NC_015555:424856-425131 [Thermoanaerobacterium xylanolyticum LX-11]
GTGGGCGAGACAAAAAGAATACTCGTCAGTTTGCCGGAAAGCTTACTGGAAGAAGTTGATGTACTTGCAGCTATGGAAAA
TAGAAACCGCAGTGAATTTATAAGAGAAGCCATGAAATTGTACATTAAAGAGAGAAAGAAGATGAAGATAAGGGAGAGCA
TGAAGAAAGGGTACCTTGAGATGGCGGCTATAAATGCAGAACTTGCGGAATTGGGCCTAACCGCTGACAACGAATGCTTT
AACGGATACGAAAAGAAATTGAAAAAGTGTGATTGA

Structures

Toxin

• AlphaFold DB: F6BH30

Antitoxin

• AlphaFold DB: I3VUF9